Abstract:
Objective To investigate whether Fuzi polysaccharice(FPS)induce the differentiation of Peripheral blood mononuclear cells to dendritic cells (DC).
Methods Lymphocytes are removed form the monocytes derived from peripheral blood of patients with hepatocellular carcinoma.High concentration, medium concentration and low concentration of PBS were added into test groups, GM-CSF, IL-4, TNF-αwere added into the positive contrast group and nothing were added into in negative contrast group.In ten days, the amounts of cells were caculated, the shape of cells were observed dynamiely by inverted microscope, cells were scanned by scaning electron microscope, and the surface types of DC(CD80, CD83, CD86) were tested by flow cytometer.
Results Medium concentration group of FPS could induce the differentiation of Peripheral blood mononuclear cells to DC, promote the proliferation of DCs and the expression of CD80, CD86 and CD83. These are no difference between positive contrast group and medium concentration group of FPS.But there are Significantly difference from medium concentration group of FPS to high concentration group of FPS, low concentration group of FPS and negative contrast group.
Conclusion FPS could induce the differentiation of Peripheral blood mononuclear cells to dendritic cells (DC), promote the proliferation of DC and the expression of mature surface molecule.