消癌平注射液下调MMP-9基因表达抑制卵巢癌细胞Caov-3迁移机制的研究

王淳; 董秀; 王梅; 王晓波

doi:10.3969/j.issn.1000-8179.2012.13.003

消癌平注射液下调MMP-9基因表达抑制卵巢癌细胞Caov-3迁移机制的研究

Xiaoaiping Injection Inhibits Cell Migration by Reducing MMP-9 Gene Expression in Human Ovarian Cancer Cells

摘要

摘要:
目的研究中药消癌平注射液下调MMP-9基因表达, 抑制卵巢癌细胞Caov-3迁移的机制。
方法利用消癌平注射液干预体外培养的卵巢癌Caov-3细胞, 分别应用: 细胞划痕实验观察细胞的迁移水平; 肿瘤细胞侵袭实验(Transwell 小室法)观察细胞侵袭能力; RT-PCR实验检测卵巢癌细胞中金属蛋白酶MMP-9基因表达水平。以金属蛋白酶MMPs特异性抑制剂-Doxycyclin作为阳性对照, 同时应用PBS作空白对照。
结果消癌平可有效抑制Caov-3细胞迁移, 与空白对照组相比差异有统计学意义(P < 0.05), 但与Doxycyclin组差异无统计学意义(P > 0.05);消癌平还可部分抑制血清诱导的Caov-3细胞穿过Transwell小室, 其抑制效果与Doxycyclin相似; 此外, 消癌平还能够下调MMP-9基因表达水平。
结论消癌平下调MMP-9基因表达, 抑制卵巢癌Caov-3细胞迁移。

Abstract:
Objective To study the inhibitory mechanism of an injection of the Chinese medicine Xiaoaiping (XAP) on the migration of human ovarian cancer Caov-3 cells via reduced matrix metalloproteinase-9 (MMP-9) gene expression
Methods The ovarian cancer Caov-3 cells were treated with XAP in vitro. The inhibitor doxycyclin was also applied to the MMPs as the positive control, whereas phosphate-buffered saline served as the blank control. Wound healing and cell invasion assays using Transwell chambers were used to investigate the antimetastatic activities of XAP. The MMP-9 expression was detected via real-time polymerase chain reaction and Western blot analysis.
Results XAP effectively inhibited Caov-3 cell migration and invasion and decreased the MMP-9 gene and protein expression levels (P < 0.05). Moreover, the inhibitory effect of XAP was similar to that of doxycyclin (P > 0.05).
Conclusion XAP inhibits Caov-3 cell migration by decreasing the MMP-9 expression.

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