Abstract:
Objective To explore the correlations of single nucleotide polymorphisms (SNPs) in the excision repair cross-complementation group 1 (ERCC1) gene with the development and progression of gastric carcinoma (GC).
Methods A total of 452 gastric carcinoma patients in Fujian province and 469 cancer-free controls in a population frequency-matched by age and sex were included in this study. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to determine the genotype of three SNPs, including rs11615 T > C, rs2298881 C > A, and rs3212930 T > C. The correlations of these polymorphisms with the susceptibility to gastric cancer were evaluated.
Results Compared with the TT genotype, rs11615 T > C with the C allele lowered the risk of GC (OR = 0.49, 95 % CI: 0.52~0.47, P = 0.01), indicating some protective effect on GC risk. However, rs11615 T > C with the C allele caused a higher risk for diffused GC, signifying poor prognosis (OR = 1.68, 95 % CI = 0.76~0.61, P = 0.048). SHEsis software analysis revealed that the haplotype C-C-C based on rs11615 T > C, rs2298881 C > A, and rs3212930 T > C polymorphisms lowered the risk of GC, with OR = 0.729 (95 % CI: 0.531~1.001, P = 0.049 9). In contrast, the haplotype T-C-T had a dangerous effect on GC, with OR = 1.321 (95 % CI: 1.063~1.641, P = 0.011 8).
Conclusion The rs11615 SNP of the ERCC1 gene contributes to the susceptibility to GC. The interactions among rs11615 T > C, rs2298881, and rs3212930 T > C had synergistic effects on GC.