刘勇, 韩涛, 潘源, 梁寒. PI3K信号通路对胃癌细胞P27磷酸化蛋白分布表达的影响[J]. 中国肿瘤临床, 2012, 39(14): 945-948. DOI: 10.3969/j.issn.1000-8179.2012.14.002
引用本文: 刘勇, 韩涛, 潘源, 梁寒. PI3K信号通路对胃癌细胞P27磷酸化蛋白分布表达的影响[J]. 中国肿瘤临床, 2012, 39(14): 945-948. DOI: 10.3969/j.issn.1000-8179.2012.14.002
shu
Yong LIU, Tao HAN, Yuan PAN, Han LIANG. Effects of PI3K Signal Pathway on the Distribution and Expression of Phosphorylated P27 in Gastric Cancer Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(14): 945-948. DOI: 10.3969/j.issn.1000-8179.2012.14.002
Citation: Yong LIU, Tao HAN, Yuan PAN, Han LIANG. Effects of PI3K Signal Pathway on the Distribution and Expression of Phosphorylated P27 in Gastric Cancer Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(14): 945-948. DOI: 10.3969/j.issn.1000-8179.2012.14.002
shu

PI3K信号通路对胃癌细胞P27磷酸化蛋白分布表达的影响

Effects of PI3K Signal Pathway on the Distribution and Expression of Phosphorylated P27 in Gastric Cancer Cells

    摘要
  • 摘要:
      目的  研究PI3K信号通路对胃癌BGC-823细胞中P27与磷酸化P27(p-P27)蛋白表达分布的影响。
      方法  LY294002处理BGC-823细胞, 流式细胞术测定处理前后两组细胞周期分布。Western blotting测定两组细胞总蛋白、胞浆蛋白、核蛋白中P27与p-P27(Thr187、Thr157及Ser10)蛋白含量及分布特点。
      结果  处理后G1期细胞数明显增加(83.9%vs.67.6%, P < 0.01)P27在总蛋白、胞浆蛋白和核蛋白中表达均显著增加(P < 0.01, P < 0.01, P < 0.01);p-P27(Ser10)在总蛋白、胞浆蛋白和核蛋白中表达均显著下降(P < 0.01, P < 0.01, P < 0.01);p-P27(Thr157)在总蛋白、胞浆蛋白中表达均显著下降(P < 0.01, P < 0.01), 在核蛋白中下降不明显(P=0.482);p-P27(Thr187)在总蛋白、胞浆蛋白中表达均下降, 在核蛋白中表达增高, 均无显著性差异(P=0.254, P=0.70, P=0.223)。
      结论  阻断PI3K通路可增加胃癌细胞P27蛋白表达, 降低p-P27蛋白表达, 改变P27和p-P27蛋白的核浆分布, 可以使细胞周期停滞于G1期, 诱导细胞凋亡。

     

    Abstract:
      Objective  To investigate the effects of PI3K pathway inhibition on the expression and distribution of P27 and phosphorylated P27(p-P27) in the BGC-823 gastric cancer cell line.
      Methods  The cell line BGC-823 was cultivated and treated with LY294002, a PI3K pathway inhibitor.The cell cycle was determined using flow cytometry.The total cell, cytoplasmic, and nuclear expression of P27, p-P27(Thrl87), p-P27(Thrl57), and p-P27(SerlO) were analyzed by Western blot analysis.
      Results  Inhibition of the PI3K pathway arrested cell cycle progression in the G1 phase(treated cells vs.control cells was 83.9%vs.67.6%, P < 0.01).The total cell, cytoplasmic, and nuclear expression of P27 was significantly increased in the treated group compared with those in the control group(P < 0.01, P < 0.01, and P < 0.01, respectively).The total cell, cytoplasmic, and nuclear expression of p-P27(Ser10) was significantly decreased in the treated group compared with that in the control group(P < 0.01, P < 0.01, and P < 0.01, respectively). The total cell and cytoplasmic expression of p-P27(Thr157) was significantly decreased in the treated group compared with that in the control group(P < 0.01 and P < 0.01, respectively).The nuclear expression of p-P27(Thr157) was decreased, but it did not reach statistical significance(P= 0.482).The total cell and cytoplasmic expression of p-P27(Thr187) was decreased but the nuclear expression was increased.However, the differences were not statistically significant(P= 0.254, P= 0.70, and P= 0.223, respectively).
      Conclusion  Inhibiting the PI3K pathway in the BGC-823 gastric cancer cell line upregulates P27 expression and downregulates p-P27 expression. Moreover, the cytoplasmic and nuclear distribution of P27 and p-P27 were altered.Inhibiting the PI3K pathway induces G 1 arrest and triggers the apoptosis of gastric cancer cells.

     

    • HTML全文
    • 参考文献(16)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回