MYCN基因表达变化增强神经母细胞瘤细胞SK-N-BE(2)凋亡

Induction of Apoptosis in Neuroblastoma Cell Line SK-N-BE(2) by Modified Expression of MYCN Gene

摘要:
目的以MYCN基因高表达神经母细胞瘤细胞株SK-N-BE(2)为研究对象, 改变神经母细胞瘤细胞中MYCN基因的表达水平, 观察对肿瘤细胞凋亡的影响。
方法分别构建MYCN高表达载体和MYCN-siRNA, 并转染神经母细胞瘤细胞株SK-N-BE(2), Western blot检测MYCN蛋白表达变化, 使用ELISA法检验肿瘤细胞凋亡情况。
结果 Western-blot结果显示转染MYCN高表达载体后SK-N-BE(2)细胞中MYCN蛋白表达显著增加(P < 0.05), 转染MYCN-siRNA后MYCN蛋白表达显著下降(P < 0.05)。肿瘤细胞在MYCN表达改变后, 凋亡率均显著增加。
结论神经母细胞瘤细胞株SK-N-BE(2)中MYCN基因表达抑制或增加均可诱导肿瘤细胞凋亡。

Abstract:
Objective To investigate the impact of MYCN expression on tumor apoptosis, and to modify its expression in MYCN-amplified neuroblastoma cell line.
Methods MYCN expression plasmid and MYCN siRNA were employed to modify the expression of MYCN in SK-N-BE (2) cells. Protein expression was detected by Western blot. Cell apoptosis after transfection was measured by ELISA.
Results The transfection of pcDNA3.1 (+) -MYCN increased the expression of MYCN gene, whereas the transfection of MYCNsiRNA inhibited the expression of MYCN gene. DNA fragmentation of transfected cells was higher than that of control group (P < 0.05).
Conclusion The modification of MYCN expression in SK-N-BE (2) cells can induce tumor cell apoptosis.