徐仙赟, 黄海金, 潘明倩, 廖琴, 陈明初, 张文娟, 刘潜. 普萘洛尔对小鼠血管瘤细胞体外增殖及凋亡的影响[J]. 中国肿瘤临床, 2012, 39(15): 1017-1019. DOI: 10.3969/j.issn.1000-8179.2012.15.005
引用本文: 徐仙赟, 黄海金, 潘明倩, 廖琴, 陈明初, 张文娟, 刘潜. 普萘洛尔对小鼠血管瘤细胞体外增殖及凋亡的影响[J]. 中国肿瘤临床, 2012, 39(15): 1017-1019. DOI: 10.3969/j.issn.1000-8179.2012.15.005
shu
Xian yun XU, Hai jin HUANG, Ming qian PAN, Qin LIAO, Ming chu CHEN, Wen juan ZHANG, Qian LIU. Effects of Propranolol on Mouse Hemangioendothelioma Endothelial Cell Proliferation and Apoptosis[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1017-1019. DOI: 10.3969/j.issn.1000-8179.2012.15.005
Citation: Xian yun XU, Hai jin HUANG, Ming qian PAN, Qin LIAO, Ming chu CHEN, Wen juan ZHANG, Qian LIU. Effects of Propranolol on Mouse Hemangioendothelioma Endothelial Cell Proliferation and Apoptosis[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1017-1019. DOI: 10.3969/j.issn.1000-8179.2012.15.005
shu

普萘洛尔对小鼠血管瘤细胞体外增殖及凋亡的影响

Effects of Propranolol on Mouse Hemangioendothelioma Endothelial Cell Proliferation and Apoptosis

    摘要
  • 摘要:
      目的  通过β受体阻滞剂普萘洛尔作用于小鼠EOMA血管瘤细胞体外增殖及凋亡的实验研究, 初步探讨普萘洛尔治疗血管瘤的机制。
      方法  体外培养EOMA细胞, 使用不同浓度普萘洛尔分别作用于EOMA细胞24、36、48 h, 应用噻唑蓝(MTT)法检测细胞存活率、吖啶橙染色检测细胞凋亡情况, 观察普萘洛尔对EOMA细胞体外增殖和凋亡的影响。
      结果  作用24 h后, 随剂量增加, EOMA细胞存活率逐渐下降, 与对照组相比, 至药物浓度为75μmol/L时有显著差异(P < 0.05), 继续增加至800μmol/L时, 细胞存活率接近0, 同时吖啶橙染色示凋亡细胞逐渐增多, 与对照组相比, 至药物浓度75μmol/L时, 细胞凋亡率有显著差异(P < 0.05)。36 h组和48 h组变化趋势与24h组相似。
      结论  普萘洛尔在体外可有效抑制小鼠EOMA血管瘤细胞的增殖并促进其凋亡, 这一作用呈现明显的剂量-时间效应依赖性。

     

    Abstract:
      Objective  To explore the primary mechanism ofpropranolol treatment on hemangioma.
      Methods  Mouse hemangioendothelioma endothelial (EOMA) cells cultured in vitro were used as the cell models in our study. Cells were treated using propranolol at different concentrations (5 μmol/L to 800 μmol/L) for 24 h to 48 h. Cell proliferation was analyzed by MTT assay, and apoptosis was studied by acridine orange (AO) staining.
      Results  After 24 h to 48 h treatment, significant differences of cell viability and apoptosis were noted (P < 0.05) at the concentration of 75 μmol/L compared with the control group (0 μmol/L). When the dose was increased, cell viability dropped, whereas apoptosis dramatically increased.
      Conclusion  Propranolol can effectively inhibit the proliferation and induce the EOMA cell apoptosis in vitro.

     

    • HTML全文
    • 参考文献(9)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回