刘勇刚, 陈汝福, 于新发, 周成宇, 李志花. 阻断NF-κB信号通路对人肝门部胆管癌细胞QBC939增殖的影响[J]. 中国肿瘤临床, 2012, 39(15): 1065-1068. DOI: 10.3969/j.issn.1000-8179.2012.15.018
引用本文: 刘勇刚, 陈汝福, 于新发, 周成宇, 李志花. 阻断NF-κB信号通路对人肝门部胆管癌细胞QBC939增殖的影响[J]. 中国肿瘤临床, 2012, 39(15): 1065-1068. DOI: 10.3969/j.issn.1000-8179.2012.15.018
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Yong gang LIU, Ru fu CHEN, Xin fa YU, Cheng yu ZHOU, Zhi hua LI. Effect of NF-κB Pathway Interference on the Proliferation of Human Hilar Cholangiocarcinoma QBC939 Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1065-1068. DOI: 10.3969/j.issn.1000-8179.2012.15.018
Citation: Yong gang LIU, Ru fu CHEN, Xin fa YU, Cheng yu ZHOU, Zhi hua LI. Effect of NF-κB Pathway Interference on the Proliferation of Human Hilar Cholangiocarcinoma QBC939 Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1065-1068. DOI: 10.3969/j.issn.1000-8179.2012.15.018
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阻断NF-κB信号通路对人肝门部胆管癌细胞QBC939增殖的影响

Effect of NF-κB Pathway Interference on the Proliferation of Human Hilar Cholangiocarcinoma QBC939 Cells

    摘要
  • 摘要:
      目的  研究阻断NF-κB信号通路对人肝门部胆管癌细胞QBC939生长增殖的影响。
      方法  常规培养QBC939细胞, 采用PDTC(100μmol/L)阻断NF-κB信号通路, Western blot检测核内P65蛋白水平的表达, CCK-8检测细胞生长增殖抑制率, 流式细胞仪观察细胞周期与凋亡的变化情况。
      结果  经PDTC阻断NF-κB信号通路后, 与对照组相比, 细胞核内P65蛋白水平明显下降(P < 0.05)。CCK-8检测显示细胞增殖活性明显受到抑制, 并随时间的延长而愈渐明显, 12、24、36h后细胞增殖抑制率分别为(22.53±0.03)%、(46.54±0.03)%、(58.02±0.03)%, 与对照组相比差异有统计学意义(P < 0.05)。流式细胞仪检测发现实验组G0/G1期细胞比例高于对照组, 分别为(68.53±1.72)%、(38.3±1.35)%, 差异有统计学意义(P < 0.05);细胞凋亡率在实验组为(27.68±2.77)%, 对照组仅为(9.27±2.36)%, 差异有统计学意义(P < 0.05)。
      结论  阻断NF-κB信号通路诱导人肝门部胆管癌细胞G0/G1期阻滞及细胞凋亡, 从而抑制细胞生长与增殖, 提示NF-κB信号通路组成性激活参与人肝门部胆管癌的发生发展, 以NF-κB信号通路作为治疗的靶点可能给人肝门部胆管癌带来新的治疗选择。

     

    Abstract:
      Objective  To evaluate the effect of NF-kB pathway interference on the proliferation of QBC939 cells.
      Methods  Human hilar cholangiocarcinoma QBC939 cells were cultured in RPMI1640 medium, and NF-kB activity was suppressed by PDTC (100 μmol/L). The P65 subunits of NF-kB in the nuclei of QBC939 cells were detected by Western blot analysis. The inhibition rates of cell growth were assayed by the CCK-8 method. Cell cycles and cell apoptosis were analyzed by flow cytometry (FCM).
      Results  The p65 subunits of NF-kB decreased significantly after PDTC (100 μmol/L) treatment (P < 0.05). The CCK-8 assay showed that the proliferation of QBC939 cells was significantly suppressed by PDTC in a time-dependent manner (P < 0.05).The inhibition rates were 22.53%±0.03%, 46.54%±0.03%, and 58.02%±0.03% after 12, 24, and 36 h, respectively. Cell cycle analysis by FCM showed that the percentage of QBC939 cells in the G0/G1 phase was increased by PDTC treatment, i.e., 68.53%±1.72% vs. 38.3%±1.35% (P < 0.05). PDTC induced 27.68% ±2.77% apoptosis compared with 9.27%±2.36% in the controls (P < 0.05).
      Conclusion  NF-kB functions in the cell proliferation and survival of human hilar cholangiocarcinoma QBC939 cells. The inhibition of NF-kB activity induces cell apoptosis and cell cycle arrest in the G0/G1 phase. Thus, targeting the NF-kB signaling pathway can be a new therapeutic strategy for human hilar cholangiocarcinoma.

     

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