李振华, 田子强, 丛斌, 张月峰, 温士旺, 李勇. 食管癌组织中MT-1-2与-3的表达差异及临床意义[J]. 中国肿瘤临床, 2012, 39(15): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2012.15.024
引用本文: 李振华, 田子强, 丛斌, 张月峰, 温士旺, 李勇. 食管癌组织中MT-1-2与-3的表达差异及临床意义[J]. 中国肿瘤临床, 2012, 39(15): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2012.15.024
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Zhen hua LI, Zi qiang TIAN, Bin CONG, Yue feng ZHANG, Shi wang WEN, Yong LI. Expression of MT-1, -2, and -3 in Esophageal Cancer and Their Clinical Significances[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2012.15.024
Citation: Zhen hua LI, Zi qiang TIAN, Bin CONG, Yue feng ZHANG, Shi wang WEN, Yong LI. Expression of MT-1, -2, and -3 in Esophageal Cancer and Their Clinical Significances[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1091-1095. DOI: 10.3969/j.issn.1000-8179.2012.15.024
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食管癌组织中MT-1-2与-3的表达差异及临床意义

Expression of MT-1, -2, and -3 in Esophageal Cancer and Their Clinical Significances

    摘要
  • 摘要:
      目的  金属硫蛋白(metallothionein, MT)是一类广泛存在于生物体体内的低分子量、富含半胱氨酸的蛋白质。本研究旨在明确MT-1、-2和MT-3在食管癌中的表达差异以及MT-1、-2和MT-3的表达量与各临床病理特征的关系。
      方法  随机选取2008年7月至2009年7月河北医科大学第四医院胸外科行手术治疗的食管癌患者114例, 其中, 男64例, 女50例; 年龄40~75岁, 平均年龄61岁。胸上段食管癌21例, 肠中段食管癌58例, 肠下段食管癌35例。依据国际抗癌联盟(UICC)食管癌TNM分期(2009年): Ⅱa期34例, Ⅱb期36例, Ⅲ期31例, Ⅳ期13例。组织类型均为鳞癌; 组织学分级为高分化59例, 中分化癌36例, 低分化癌19例。应用Western blot检测肿瘤组织及切缘正常组织中MT-1、-2和MT-3的表达情况, 对MT-1、-2和MT-3的表达量与临床病理参数, 以及MT-1、-2与MT-3表达的关系进行分析。
      结果  MT-1、-2和MT-3在食管癌组织中的表达量均高于切缘正常组织(MT-1、-2 t=5.214, P < 0.01), (MT-3 t=4.287, P < 0.01), 且均与肿瘤组织病理分期(MT-1、-2 rs=0.896, P < 0.01), (MT-3 rs=-0.501, P < 0.01), 分化程度(MT-1、-2 rs=-0.548, P < 0.01), (MT-3 rs=0.664, P < 0.01)有关, 与肿瘤部位(MT-1、-2 rs=0.253, P > 0.05), (MT-3 rs=0.172, P > 0.05)无关。
      结论  MT-1、-2和MT-3在食管癌组织中均存在高表达, 其表达水平与食管癌的发生、发展及组织分化密切相关, 而且MT-3在食管癌组织中的表达规律与MT-1, -2有显著不同。

     

    Abstract:
      Objective  To determine the expression differences of MT-1, -2, and -3 in esophageal cancer, as well as the relationship of these differences with the clinicopathological parameters.
      Methods  A total of 114 esophageal cancer patients were randomly selected from the Department of Thoracic Surgery of The Fourth Affiliated Hospital of Hebei Medical University from July 2008 to July 2009. The patients included 64 males and 50 females aged 40-75 years (mean = 61 years). The tumor was located in the upper third of the esophagus for 21 cases, in the middle third for 58 cases, and in the lower third for 35 cases. Based on the UICC TNM staging of esophageal cancer (2009), there were 34, 36, 31, and 13 cases of stages IIa, IIb, III, and IVa, respectively. All were squamous cell carcinoma with 59, 36, and 19 cases of well-, moderately, and poorly differentiated carcinoma, respectively. The entire cell protein of these tissues was extracted, and the expression of MT-1, -2, and -3 was detected by Western blot analysis. MT-1, -2, and -3 expression in normal esophageal tissue was compared with that in tumor tissue. The correlation of MT expression with the clinicopathological parameters was examined. The correlation of the expression of MT-3 with those of MT-1 and-2 were also analyzed.
      Results  The expression of MT-1, -2, and -3 was higher in esophageal cancer tissues than in adjacent normal tissues (MT-1 and -2: t= 5.214, P < 0.01; MT-3: t= 4.287, P < 0.01). The expression of MT-1, -2, and -3 in tumor tissue was associated with the tumor pathological stage (MT-1 and-2: rs= 0.896, P < 0.01; MT-3: rs=-0.501, P < 0.01) and tumor differentiation MT-1 and-2: rs =-0.548, P < 0.01; MT-3: rs = 0.664, P < 0.01), but was not closely related with the tumor site (MT-1 and -2: rs= 0.253, P > 0.05; MT-3: rs= 0.172, P > 0.05).
      Conclusion  The expression levels of MT-1 to -3 in esophageal cancer tissue were significantly higher than in adjacent normal tissue. This high expression was closely related with the occurrence, development, and differentiation of esophageal carcinoma. The expression of MT-3 obviously differed from that of MT-1 and -2 in esophageal cancer.

     

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