黄文炼, 徐曼, 王婷婷, 耿卫朴, 陈瑜. 乳腺癌B7-H4异常表达及与肿瘤内CD8+T细胞浸润IFN-γ分泌的相关性研究[J]. 中国肿瘤临床, 2012, 39(15): 1096-1099, 1103. DOI: 10.3969/j.issn.1000-8179.2012.15.025
引用本文: 黄文炼, 徐曼, 王婷婷, 耿卫朴, 陈瑜. 乳腺癌B7-H4异常表达及与肿瘤内CD8+T细胞浸润IFN-γ分泌的相关性研究[J]. 中国肿瘤临床, 2012, 39(15): 1096-1099, 1103. DOI: 10.3969/j.issn.1000-8179.2012.15.025
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Wen lian HUANG, Man XU, Ting ting WANG, Wei po GENG, Yu CHEN. Over Expression of B7-H4 in Breast Carcinoma Inversely Correlates with Cellular Infiltration of CD8+T Cells and IFN-γ Secretion[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1096-1099, 1103. DOI: 10.3969/j.issn.1000-8179.2012.15.025
Citation: Wen lian HUANG, Man XU, Ting ting WANG, Wei po GENG, Yu CHEN. Over Expression of B7-H4 in Breast Carcinoma Inversely Correlates with Cellular Infiltration of CD8+T Cells and IFN-γ Secretion[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(15): 1096-1099, 1103. DOI: 10.3969/j.issn.1000-8179.2012.15.025
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乳腺癌B7-H4异常表达及与肿瘤内CD8+T细胞浸润IFN-γ分泌的相关性研究

Over Expression of B7-H4 in Breast Carcinoma Inversely Correlates with Cellular Infiltration of CD8+T Cells and IFN-γ Secretion

    摘要
  • 摘要:
      目的  探讨B7同源体4(B7 homolog 4, B7-H4)在人乳腺癌组织的表达及其与肿瘤内CD4+T、CD8+T细胞的数量和IFN-γ细胞因子分泌的关系。
      方法  应用S-P免疫纽织化学染色检测B7-H4在乳腺癌、癌旁组织和乳腺良性病变组织中的表达; 间接免疫荧光双标染色分析乳腺癌组织内浸润的CD4+T、CD8+T细胞数量及TGF-β1和IFN-γ分泌情况。
      结果  B7-H4在乳腺癌、乳腺良性病变和癌旁组织的阳性表达率分别为75.0%、37.5%和25.0%, 三组间存在显著性差异(均P < 0.01);B7-H4阳性乳腺癌组织内浸润的CD8+T细胞及IFN-γ分泌细胞数量明显低于B7-H4阴性的乳腺癌组织(P < 0.05), 但B7-H4的表达与乳腺癌组织内浸润的CD4+T细胞及TGF-β1分泌细胞数量无关。
      结论  B7-H4在乳腺癌中表达明显上调, 并与抑制CD8+T细胞浸润和分泌IFN-γ有关。B7-H4在下调乳腺癌抗肿瘤细胞免疫中具有一定作用。

     

    Abstract:
      Objective  To investigate the B7-H4 expression in human breast carcinoma and analyze its relationship with the number of tumor-infiltrating CD4+T and CD8+T lymphocytes as well as cytokine secretion.
      Methods  B7-H4 expression was detected through immunohistochemistry in 72 cases of breast cancer, 16 cases of benign breast lesions, and 16 cases of normal breast tissues. The number of tumor-infiltrating CD4+T and CD8+T lymphocytes as well as TGF-β1 and IFN-Υ secretion was determined by indirect immunofluorescence double-staining.
      Results  B7-H4 was expressed in 75% of breast cancer cases, with the positive rate significantly higher than that of benign lesions and normal breast tissues (P < 0.01 and P < 0.01, respectively). In B7-H4 positive breast carcinomas, the number of infiltrating CD8+T lymphocytes and IFN-Υ, secreting cells was significantly lower than that of B7-H4 negative breast carcinomas (P < 0.05). However, no difference was observed in the number of infiltrating CD4+T lymphocytes and TGF-31 producing cells between these two groups.
      Conclusion  B7-H4 is over expressed in breast cancer and is associated with decreasing CD8+T lymphocyte infiltration and IFN-7 secretion. BT-H4 plays a significant role in depressing anti-tumor T cell immunity of breast cancer.

     

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