王静萱, 张清媛, 刘姝伶, 武海燕, 赵文辉, 卢康平. 乳腺癌组织中YY1表达与E-cadherin甲基化状态的关系[J]. 中国肿瘤临床, 2012, 39(16): 1169-1171+1179. DOI: 10.3969/j.issn.1000-8179.2012.16.009
引用本文: 王静萱, 张清媛, 刘姝伶, 武海燕, 赵文辉, 卢康平. 乳腺癌组织中YY1表达与E-cadherin甲基化状态的关系[J]. 中国肿瘤临床, 2012, 39(16): 1169-1171+1179. DOI: 10.3969/j.issn.1000-8179.2012.16.009
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Jingxuan WANG, Qingyuan ZHANG, Shuling LIU, Haiyan WU, Wenhui ZHAO, Kangping LU. Association between YYl Methylation of E-cadherin in Breast Cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(16): 1169-1171+1179. DOI: 10.3969/j.issn.1000-8179.2012.16.009
Citation: Jingxuan WANG, Qingyuan ZHANG, Shuling LIU, Haiyan WU, Wenhui ZHAO, Kangping LU. Association between YYl Methylation of E-cadherin in Breast Cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(16): 1169-1171+1179. DOI: 10.3969/j.issn.1000-8179.2012.16.009
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乳腺癌组织中YY1表达与E-cadherin甲基化状态的关系

Association between YYl Methylation of E-cadherin in Breast Cancer

    摘要
  • 摘要:
      目的  探讨乳腺癌组织中YY1(Yin Yang-1, YY1)的表达与E-cadherin甲基化状态之间的关系。
      方法  采用免疫组织化学法检测42例乳腺癌原发病灶以及18例正常乳腺组织中YY1的表达情况, 采用甲基化特异性聚合酶链反应法(MSP)进行E-cadherin基因甲基化检测。
      结果  乳腺癌中E-cadherin甲基化率为57.1%(24/42), 显著高于正常乳腺组织的11.1%(2/18), 差异有统计学意义(P < 0.05)。乳腺癌组织中阳性YY1的表达(22/42, 52.3%)显著高于正常乳腺组织(2/18, 16.7%), 差异有统计学意义(P < 0.05), YY1阳性乳腺癌组中E-cadherin甲基化者为20/22(90.9%), 而YY1阴性组的乳腺癌E-cadherin甲基化者为4/16(25%), YY1与E-cadherin甲基化状态之间呈正相关(P < 0.05)。
      结论  乳腺癌患者E-cadherin基因启动子甲基化发生率显著高于正常乳腺组织, 乳腺癌组织中YY1的高表达有可能是E-cadherin甲基化失活的主要原因。

     

    Abstract:
      Objectives  To determine the relationship between the methylation of the E-cadherin promoter region and YY1 expression in breast cancer.
      Methods  YY 1 expression was detected in the primary cancer tissues of 42 breast cancer patients and the normal breast tissues of 18 patients with benign breast tissues through immunohistochemical staining. E-cadherin gene methylation was examined using methylation-specific polymerase chain reaction (MSP).
      Results  The rate of E-cadherin gene hypermethylation was 57.1% (24 / 42), which is significantly higher than that in normal breast tissues (11.1%; 2 / 18) (P < 0.05). The rate of positive YY 1 expression in breast cancer lesions (52.3 %; 22 / 42) was significantly higher than in normal breast tissues (16.7 %; 2 / 18) (P < 0.05). Hypermethylation of E-cadherin gene promoter was detected in 88 % (22 / 25) of YYl-positive cases and in 11.4 % (4 / 35) of YYl-negative cases. The difference in the methylation of the E-cadherin promoter was significantly higher among YY 1-positive patients than among YY 1-negative patients (P < 0.05).
      Conclusion  Methylation of E-cadherin gene promoter is a common molecular event in breast cancer and YY1 may be involved in the methylated regulation of E-cadherin.

     

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