李秀娟, 聂永梅, 张志强, 张桂英, 李茂玉, 宋娜, 苏丽萍, 倪自翔. S100A9蛋白在胃癌中的表达及其临床病理意义[J]. 中国肿瘤临床, 2012, 39(16): 1188-1191. DOI: 10.3969/j.issn.1000-8179.2012.16.014
引用本文: 李秀娟, 聂永梅, 张志强, 张桂英, 李茂玉, 宋娜, 苏丽萍, 倪自翔. S100A9蛋白在胃癌中的表达及其临床病理意义[J]. 中国肿瘤临床, 2012, 39(16): 1188-1191. DOI: 10.3969/j.issn.1000-8179.2012.16.014
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Xiujuan LI, Yongmei NIE, Zhiqiang ZHANG, Guiying ZHANG, Maoyu LI, Na SONG, Liping SU, Zixiang NI. Expression and Clinicopathologic Significance of S100A9 Protein in Gastric Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(16): 1188-1191. DOI: 10.3969/j.issn.1000-8179.2012.16.014
Citation: Xiujuan LI, Yongmei NIE, Zhiqiang ZHANG, Guiying ZHANG, Maoyu LI, Na SONG, Liping SU, Zixiang NI. Expression and Clinicopathologic Significance of S100A9 Protein in Gastric Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(16): 1188-1191. DOI: 10.3969/j.issn.1000-8179.2012.16.014
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S100A9蛋白在胃癌中的表达及其临床病理意义

Expression and Clinicopathologic Significance of S100A9 Protein in Gastric Carcinoma

    摘要
  • 摘要:
      目的  探讨胃癌(GC)中S100A9的表达及临床病理意义。
      方法  蛋白质组织学定量鉴定GC和癌旁胃黏膜(GM)组织的差异表达蛋白质; Western-blot验证蛋白S100A9的表达; 免疫组织化学检测S100A9蛋白在GC、GM和原发性胃癌转移的淋巴结(LN)的表达; 分析S100A9表达与GC临床病理参数的关系。
      结果  蛋白质组织学筛选的78个差异蛋白中, S100A9表达在GC中较正常GM中明显上调(1∶0.09), Western-blot验证S100A9蛋白在GC表达上调(P < 0.01);免疫组织化学显示: 70例正常GM中S100A9阳性表达23例(32.86%); 118例GC中S100A9阳性表达72例(61.02%); 35例LN中S100A9阳性表达29例(82.86%), 与正常GM相比, GC和LN中S100A9的表达明显升高(P < 0.01);S100A9在LN的GC中上调(P < 0.05);S100A9表达与GC的淋巴结转移、分化程度、浸润深度和TNM分期相关(P < 0.01或P < 0.05), 而与患者的年龄和性别无关(P < 0.05)。
      结论  S100A9高表达与GC发生发展、侵袭转移有关, 可能是GC的一个促癌因子。

     

    Abstract:
      Objective  This study aims to investigate the expression and clinicopathologic significance of the S 100A9 protein in gastric cancer (GC).
      Methods  Differential expression of proteins between GC and paraneoplastic gastric mucosa (GM) tissues was quantitatively determined using proteomics. The S100A9 protein level was examined using western blot analysis. The S 100A9 protein expression in GC, GM, and lymph node (LN) of primarily gastric carcinomatous metastasis was determined using immunohistochemistry (IHC). The relationship between S100A9 protein expression and the clinicopathologic parameters of GC was analyzed.
      Results  Among the 78 differential proteins identified through proteomics, S 100A9 protein expression was clearly upregulated in GC compared with the GM (1:0.09), which was confirmed through western blot analysis (P < 0.01). The IHC assay showed the positive expression of 23 S100A9 proteins (32.86 %) among 70 normal GC samples, 72 (61.02 %) among 118 GM samples, and 29 (82.86 %) among 35 LN samples. Compared with GM, S100A9 expression was significantly increased in GC and LN (P < 0.01). S100A9 expression was upregulated in LN compared with GC (P < 0.05). The S100A9 expression level in GC was correlated with LN metastasis, histologic differentiation, depth of invasion, and TNM stage (P < 0.01 or P < 0.05), but not correlated with the patient age and gender (P > 0.05).
      Conclusion  S100A9 protein overexpression is correlated with GC carcinogenesis, invasion, and metastasis and it may promote GC.

     

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