Abstract:
Objective To assess the mechanism of ING1 gene silencing on cell apoptosis of gastric cancer cell line AGS.
Methods The synthetic siRNA specific to ING1 was transfected into AGS cells. Cell apoptosis was evaluated by flow cytometry and TUNEL. The expression levels of ING1, caspase 2 and caspase 3 were detected by real-time PCR and western-blot.
Results The synthetic siRNA could effectively inhibit the expression of ING1 in AGS cells. The apoptosis cells number of ING1 knocked-down group was 6.70 ± 0.41%, which was significantly decreased compared with the control group (11.06 ± 0.97%, P < 0. 05). 40 hours after transfection, the mRNA expression of ING1, caspase 2 and caspase 3 was 0.1707 ± 0.06, 0.125 ± 0.03 and 0.999 ± 0.10, respectively. The tendency of protein variation of ING1, caspase2 and caspase 3 was consistent with mRNA.
Conclusion ING1 gene silencing could down-regulated caspase 2 gene expression and ING1 plays an important role in regulating AGS cell apoptosis by down-regulated caspase 2 gene expression.