宫颈病变卡波济肉瘤相关疱疹病毒感染状况分析

姚水洪; 陈敏; 查国芬; 邱惠萍; 陆小青

doi:10.3969/j.issn.1000-8179.2012.17.006

宫颈病变卡波济肉瘤相关疱疹病毒感染状况分析

Analysis of Kaposi's Sarcoma-associated Herpesvirus Infection Status in Females with Cervical Lesions

摘要

摘要:
目的了解宫颈病变卡波济肉瘤相关疱疹病毒（KSHV）感染状况，探讨KSHV感染与宫颈病变的关系。
方法选择组织学确诊为宫颈上皮内瘤变（CIN）和宫颈癌（SCC）的病例440例作为研究组，同区域、同期就诊或体检并排除有宫颈病变的女性380例为对照组，分别采集血液和宫颈脱落细胞标本，提取DNA，巢式PCR检测KSHV ORF26，核酸电泳观察结果。
结果研究组血液样品KSHV检出率12.9%（53/412），对照组检出率6.5%（23/354），两组差异有统计学意义（P < 0.01），但宫颈脱落细胞样品中未能检出KSHV；对研究组作分层分析，KSHV检出率随年龄增加、宫颈病变程度加重而升高，差异有统计学意义（P < 0.05）。
结论宫颈病变女性KSHV检出率高于正常女性，感染率随宫颈病变程度加重而升高，应加强监测。

Abstract:
Objective To aimed to analyze the infection status of female patients with Kaposi's sarcoma-associated herpesvirus (KSHV) and its relationship with the occurrence of cervical lesions.
Methods For the study group, 440 histological cases with confirmed cervical intraepithelial neoplasia (CIN) and cervical squamous cell cancer (SCC) were randomly selected. Simultaneously, 380 cases without cervical lesions in the same region were physically examined as the control group. Blood and cervical cell samples were collected for DNA extraction, and the KSHV ORF26 was detected using nested PCR and gel electrophoresis.
Results The KSHV detection rate of blood samples in the study group was 12.9% (53/412), whereas that in the control group was 6.5% (23/354). The difference between the two groups was statistically significant (P < 0.01), but KSHV could not be detected in the cervical cell samples. Stratified analysis was applied for the study group, and the detection rate of KSHV was found to significantly increase with the aggravation of cervical lesions and with increasing age (P < 0.05).
Conclusion The KSHV detection rate in females with cervical lesions is higher than in normal females. Thus, KSHV can potentially strengthen the monitoring of cervical lesions.

HTML全文

参考文献(13)

施引文献

资源附件(0)