Abstract:
Objective To analyze the clinical distribution and the resistance status of Haemophilus isolated from the respiratory tract of patients with tumors, and supply envidence for the standard application of antibiotics in clinical practices.
Methods Chocolate agar plates containing X and V factors were used to isolate and culture Haemophilus. Vitek2-Compact was applied to identify the species. In vitro antibiotic susceptibility experiments were performed by the broth microdilution method. The cephalosporin nitrocefin disk diffusion method was used to detect β-lactamase.
Results A total of 316 isolates were detected from 7333 respiratory specimens, including 95 isolates of Haemophilus influenzae (Hi) and 221 isolates of Haemophilus parainfluenzae (Hpi). All Hi isolates were sensitive to cefotaxime, levofloxacin, and meropenem. The resistance rates to Compound Sulfamethoxazole and ampicillin were 26.3% and 15.8%, respectively. About 90% or above of the Hi isolates were sensitive to cephalosporins, β-lactamase/β-lactamase inhibitor, macrolides, and chloromycetin. The antimicrobial resistance of Hpi was similar to that of Hi.
Conclusion The majority of antibiotics maintained good antibacterial activity in cancer patients with respiratory tract infection caused by Haemophilus. The clinical application of antibiotics should be based on the results of in vitro antibiotic susceptibility testing.
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