查全斌, 张华, 唐金海, 吴建中, 季明华. 紫杉醇   顺铂对HCC1937人乳腺癌细胞MAPK信号通路影响[J]. 中国肿瘤临床, 2012, 39(19): 1397-1400. DOI: 10.3969/j.issn.1000-8179.2012.19.002
引用本文: 查全斌, 张华, 唐金海, 吴建中, 季明华. 紫杉醇   顺铂对HCC1937人乳腺癌细胞MAPK信号通路影响[J]. 中国肿瘤临床, 2012, 39(19): 1397-1400. DOI: 10.3969/j.issn.1000-8179.2012.19.002
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Quanbin CHA, Hua ZHANG, Jinhai TANG, Jianzhong WU, Minghua JI. Role of the MAPK Signal Pathway in Paclitaxel- and Cisplatin-induced Apoptosis in HCC1937 Breast Cancer Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(19): 1397-1400. DOI: 10.3969/j.issn.1000-8179.2012.19.002
Citation: Quanbin CHA, Hua ZHANG, Jinhai TANG, Jianzhong WU, Minghua JI. Role of the MAPK Signal Pathway in Paclitaxel- and Cisplatin-induced Apoptosis in HCC1937 Breast Cancer Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(19): 1397-1400. DOI: 10.3969/j.issn.1000-8179.2012.19.002
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紫杉醇   顺铂对HCC1937人乳腺癌细胞MAPK信号通路影响

Role of the MAPK Signal Pathway in Paclitaxel- and Cisplatin-induced Apoptosis in HCC1937 Breast Cancer Cells

    摘要
  • 摘要:
      目的  研究紫杉醇、顺铂对BRCA1基因缺陷型三阴性乳腺癌细胞HCC1937的增殖抑制作用及与MAPK信号通路的关系。
      方法  采用CCK-8试剂盒检测紫杉醇、顺铂分别对HCC1937细胞、MCF-7细胞的50%抑制浓度(IC50); 采用流式细胞仪和Western blot分别检测两药作用HCC1937细胞48 h后的细胞周期和MAPK通路蛋白表达状况。
      结果  HCC1937细胞(IC50 6~9μg/mL)对顺铂敏感性显著高于MCF-7细胞(IC50 18~20μg/mL)(P < 0.01);而HCC1937细胞(IC50 3~4.6μg/mL)对紫杉醇的敏感性则明显低于MCF-7细胞(IC50 0.12~0.3μg/mL)(P < 0.01)。紫杉醇使HCC1937细胞阻断在G2~M期, 呈剂量-效应趋势, 顺铂使其阻断在G0/G1期; 紫杉醇、顺铂作用HCC1937细胞48 h后, P-JNK和P-P38蛋白表达显著增加, 顺铂组P-ERK蛋白表达较对照组明显降低。
      结论  1) 三阴性乳腺癌细胞HCC1937对顺铂的敏感性明显优于紫杉醇; 2)紫杉醇、顺铂皆可激活HCC1937细胞JNK/SAPK、P38通路, 同时不同浓度的顺铂可以抑制ERK通路激活。

     

    Abstract:
      Objective  This study aimed to investigate the inhibitory effects of paclitaxel and cisplatin against the triple-negative breast cancer cell line HCC1937 in vitro, and to explore the underlying mechanisms in relation to the MAPK signaling pathway.
      Methods  A Cell Counting Kit-8 assay was used to detect the 50%inhibitory concentration(IC50) of paclitaxel and cisplatin on HCC1937 and MCF-7 cells.Flow cytometry and Western blot analysis were used to determine the cell cycle distribution and MAPK signaling pathway protein expression after incubating the HCC1937cells with paclitaxel or cisplatin for 48 h.
      Results  The HCC1937 cells were significantly more sensitive to cisplatin(IC50= 6-9μg/ml) than MCF-7 cells(IC50= 18-20μg/ml)(P < 0.01), whereas the HCC1937 cells were less sensitive to paclitaxel(IC 50= 3-4.6μg/ml) than the MCF-7 cells(IC50 = 0.12-0.3μg/ml)(P < 0.01).Paclitaxel caused the MCF-7 cells to arrest at the G 2/M phase in a concentration-dependent manner, whereas cisplatin acted by arresting cells at the G0/G1 phase.P-JNK and P-P38 protein expression significantly increased after the cells were treated with the two drugs for 48 h compared with the control group.However, P-ERK protein expression in the cisplatin group was significantly lower than in the control group for the HCC1937cells.
      Conclusion  HCC1937 cells have higher chemosensitivity to cisplatin than to paclitaxel.Paclitaxel and cisplatin can both activate the JNK/SAPK and P38 signaling pathways in HCC1937 cells, whereas different cisplatin concentrations can inhibit the activation of the ERK signaling pathway.

     

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