Abstract:
Objective This study aims to explore the molecular mechanisms in the progression of early-stage tumors by comparing and analyzing the copy number variation (CNV) in an immortalized epithelial cell line and the lung squamous cell carcinoma cell line.
Methods Human genome array-comparative genomic hybridization was used to measure the CNVs of the immortalized cell line Y-BE and the lung squamous cell carcinoma cell line NCI-H2170. An analysis of the Gene Ontology functional enrichment of the genes with abnormal copy numbers was conducted after data reconciliation.
Results Only a few variations occurred in the early passage of the Y-BE cell, Yp21. A large overlapping CNV region in 20 q11-12 between the cell Yp21 and NCI-H2170 was found. The copy-number gain of neural cadherin-like cell adhesion genes occurred in the late passage of the Y-BE cell, Yp113. Compared with NCI-H2170, the CNV frequency of Yp113 increased, whereas the genome stability from the early passage Y-BE to the late passage one decreased.
Conclusion After studying CNVs in the immortalized epithelial cell line, the CNV spectrum of variation of the precancerous model for lung cancer was successfully built. Some of the CNVs represented an early molecular event of tumor progression, indicating the potential malignancy of the cells. The findings of this study may give clues clarifying the molecular mechanisms of tumorigenesis and progression
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