Abstract:
Objective To analyze the mRNA expressions of the centrosome γ-tubulin and its regulatory factor Nek2 in high/low-grade carcinoma and in atypical ductal hyperplasia and to evaluate the mechanism of breast cancer evolution.
Methods The breast tissue samples (six groups) were collected from normal tissues, atypical hyperplasia, high- and low-grade ductal carcinoma in situ, and high- and low-grade of invasive ductal carcinoma. The mRNA expression levels of γ-tubulin and Nek2 were detected by in situ hybridization in 180 samples and by real-time qRT-PCR in 80 samples.
Results The positive rate and quantity of γ-tubulin and Nek2 mRNA expressions in high/low-grade ductal carcinoma in situ and in invasive ductal carcinoma significantly increased compared with those in normal tissues (P < 0.01). A significant difference was observed among the all six groups (χ2=37.519, χ2=36.912, P < 0.001). The difference of mRNA expression levels between any two groups of the carcinoma was not statistically significant (P>0.05). The expressions and the abnormal up-tendencies of γ-tubulin and Nek2 mRNA were not statistically significant. Comparing the positive rate of expression between the atypical ductal hyperplasia and low-grade carcinoma, the results were χ2 = 1.200, 0.659, 1.148 and 2.700, respectively (P>0.05). However, the quantitative analysis of these samples showed that statistical difference between them was significant (P < 0.01).
Conclusion The abnormal mRNA expression of γ-tubulin and Nek2 may have an important function in the successive proliferation of the malignant transformation process in mammary cellula epithelialis