Abstract:
Objective This study aims to evaluate docetaxel-sensitive and docetaxel-resistant proteome in PC-3 prostatic cancer cells, as well as the molecular mechanisms of the docetaxel-resistant PC-3 cells.
Methods Docetaxel-resistant PC-3 cells were cultured by a dose escalation of docetaxel. The global profiling of the protein expression was investigated through the docetaxel-sensitivity and drug resistance of PC-3 cells using 2-dimensional polyacrylamide gel electrophoresis, matrix-assisted laser desorption, or ionization time-of-flight mass spectrometry,
Results Compared with docetaxel-sensitive PC-3 cells, 49 more differential proteins were found in the docetaxel-resistant PC-3 cells after performing the DIGE and MALDI-TOF-TOF examinations. The expression of 29 proteins was up-regulated, whereas that of 20 proteins was down-regulated. Among these proteins, ATP synthase and galectin-1 contributed to the formation of tumor vessels, whereas Calreticulin, Cathepsin D, and Coffin contributed to tumor metastasis. Moreover, the 78 kDa glucose-regulated protein (GRP78) and microtubule-associated protein-6 were involved in the drug-resistance regulation of the tumor.
Conclusion A proteomic differential expression was observed between docetaxel-sensitive and docetaxel-resistant PC-3 cells. This finding will be helpful in understanding further the molecular mechanisms of prostate cancer invasion and drug resistance to provide new experimental evidence for the drug therapy of advanced androgen-independent prostate cancer.
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