钱敏, 王世宣, 王鸿雁, 卢运萍, 马丁, 刘荣华. BAY11-7082对前列腺癌细胞内NF-κB及其生物学行为的影响[J]. 中国肿瘤临床, 2012, 39(21): 1608-1611. DOI: 10.3969/j.issn.1000-8179.2012.21.011
引用本文: 钱敏, 王世宣, 王鸿雁, 卢运萍, 马丁, 刘荣华. BAY11-7082对前列腺癌细胞内NF-κB及其生物学行为的影响[J]. 中国肿瘤临床, 2012, 39(21): 1608-1611. DOI: 10.3969/j.issn.1000-8179.2012.21.011
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Min QIAN, Shixuan WANG, Hongyan WANG, Yunping LU, Ding MA, Ronghua LIU. Effects of BAY 11-7082 on NF-κB Expression and Its Biological Behaviors in Prostate Cancer Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(21): 1608-1611. DOI: 10.3969/j.issn.1000-8179.2012.21.011
Citation: Min QIAN, Shixuan WANG, Hongyan WANG, Yunping LU, Ding MA, Ronghua LIU. Effects of BAY 11-7082 on NF-κB Expression and Its Biological Behaviors in Prostate Cancer Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(21): 1608-1611. DOI: 10.3969/j.issn.1000-8179.2012.21.011
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BAY11-7082对前列腺癌细胞内NF-κB及其生物学行为的影响

Effects of BAY 11-7082 on NF-κB Expression and Its Biological Behaviors in Prostate Cancer Cells

    摘要
  • 摘要:
      目的  探讨BAY11-7082对前列腺癌细胞NF-κB表达及其生物学行为的影响。
      方法  应用CCK-8方法、蛋白印迹、NF-κB激活-核转运检测, 细胞增殖抑制实验和集落形成实验方法观察BAY11-7082作用下前列腺癌细胞NF-κB蛋白的表达, 细胞生长等指标的变化。
      结果  BAY11-7082对前列腺癌细胞转移有明显的抑制作用。BAY11-7082处理的前列腺癌细胞内NF-κB蛋白表达降低, 减弱NF-κB的激活, 细胞增殖和形成集落的能力下降。
      结论  BAY11-7082可抑制前列腺癌细胞NF-κB的激活与表达, 提示NF-κB可作为前列腺癌基因治疗的重要靶点之一。

     

    Abstract:
      Objective  This study was designed to determine the effect of BAY 11-7082 on NF-~cB expression and tumor colony formation of prostate cancer cells.
      Methods  Cell cytotoxicity assay kit-8 was used to detect cell death and proliferation according to the protocol. Western blot and densitometry analysis were performed to compare the expression of NF-KB in the control group with that of BAY 11-7082, an NF-nB inhibitor that is used to treat prostate cancer cells. Immunofluorescent staining was also conducted to moni- tor NF-KB activation and translocation in the nucleus. The effects of BAY 11-7082 on colony formation were determined by counting the number of prostate cancer cell colonies.
      Results  The treatment of prostate cancer cell line PC-3 cells by BAY 11-7082 inhibited cell proliferation and formed few colonies. Similarly, NF-tcB protein level dramatically decreased. Its translocation in the nucleus and the activation in the PC-3 cells were disrupted because of the effects of BAY 11-7082.
      Conclusion  BAY 11-7082 can inhibit the aberrant activation of NF-κB in prostate cancer cells and reduce tumor metastasis. Therefore, BAY 11-7082 is a potential drug for the treatment of prostate cancer patients in clinical practice in the future.

     

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