伴微乳头结构的肺腺癌恶性潜能机制研究

王静; 孙蕾娜; 闫庆娜; 田璇; 李琪; 战忠利

doi:10.3969/j.issn.1000-8179.2012.21.012

伴微乳头结构的肺腺癌恶性潜能机制研究

Epithelial-mesenchymal Transition Cell Proliferation and Cell Apoptosis in Pulmonary Adenocarcinoma with a Micropapillary Pattern

摘要

摘要:
目的探讨伴微乳头成分的肺腺癌(pulmonary adenocarcinoma with a micropapillary pattern, MPPAC)上皮间质转化和增殖调亡特征, 为临床评价其恶性潜能提供理论依据。
方法根据2011年发表的肺腺癌新分类诊断标准, 以是否伴有微小乳头状结构(micropapillary pattern, MPP)将120例肺腺癌病例分为64例MPP阳性组, 56例MPP阴性组。免疫组织化学法检测Vimentin、E-cadherin、Capase-3、pEGFR的表达。
结果 120例肺腺癌病例中pECFR在女性(P=0.035)、非吸烟(P=0.017)患者中高表达。Vimentin在MPP阳性组的表达(28.1%)高于MPP阴性组(16.1%)并且其多表达于微乳头结构腔内散落的细胞; Caspase-3在MPP阳性组高表达(P=0.001)。
结论微乳头结构的腔内散落细胞表现间质表型有利于癌细胞浸润转移, 伴乳头结构的肺腺癌有其独特的分子生物学特征。

Abstract:
Objective This paper aimed to determine the characteristics of epithelial-mesenchymal transition, cell proliferation, and cell apoptosis in pulmonary adenocarcinoma with a micropapillary pattern(MPPAC).
Methods Based on the new diagnostic criteria for lung adenocarcinoma classification(published in 2011), 120 cases of lung adenocarcinoma were divided into micropapillary pattern (MPP)-positive(n=64)and MPP-negative(n=54)groups.The expression of vimentin, E-cadherin, caspase-3, and pEGFR in pulmonary adenocarcinoma tissue specimens was determined by immunohistochemical assay.
Results pEGFR expression in 120 cases of lung adenocarcinoma was higher in female(P=0.035)and nonsmoking(P=0.017)patients.Vimentin expression was higher in the MPP-positive(28.1%)than MPP-negative(16.1%)groups, and mostly located in scattered cells in the MPP cavity.Caspase-3 expression was significantly higher in the MPP-positive group(P=0.001).
Conclusion Scattered cells in the MPP cavity have a mesenchymal phenotype, which may favor invasion and metastasis, indicating a unique characteristic of MPPAC.

HTML全文

参考文献(12)

施引文献

资源附件(0)