Abstract:
Objective To investigate the expression of nuclear factor kappa-light-chainenhancer of activated B cell (NF-κB) and Helicobacter pylori (HP) infection status with the use of gastric cancer samples and statistical analysis of HP infection status and clinical data, as well as to provide clinical diagnosis and determine gastric cancer treatment procedures.
Methods T total of 70 paraffin-embedded samples of gastric cancer and 56 normal tissues from surgically resected tissues were randomly selected. NF-κB protein expression in the samples was detected by immunohistochemical staining. A total of 8 living specimens (gastric carcinoma and corresponding normal tissues, 4 pairs of HP+, and 4 pairs of HP- were also selected. The mRNA and of NF-κB protein expression in the samples was detected by quality polymerase chain reaction and western blot.
Results The positive rates of NF-κB protein expression in HP+ gastric carcinoma tissues and adjacent nonneoplastic tissues were 82.50% and 26.32%, respectively. A significant difference in the expression levels was indicated (X2=24.87, P < 0.05, respectively). The positive rates of NF-κB protein expression in HP+ and HP- gastric carcinoma tissues were 82.50% and 53.33% respectively. These results indicated a significant difference in the expression levels (X2=6.94, P < 0.05, respectively). The NF-κB protein expression in gastric carcinoma tissues was 70.00% (49/70). A significant difference in the expression levels was indicated (P < 0.05). It correlated with tumor metastasis and invasion but not with histopathologic grading and tumor size. Compared with the adjacent nonneoplastic tissues, HP+ gastric cancer tissues exhibited higher mRNA and NF-κB protein expression in living specimens. However, no significant difference was indicated between HP+ gastric carcinoma tissues with the adjacent nonneoplastic tissues.
Conclusion NF-κB protein expression was upregulated in the HP+ gastric carcinoma sample but not in the HP- gastric cancer, HP+, or HP- adjacent nonneoplastic tissues. High NF-κB protein expression correlates with gastric cancer development.