Abstract:
Objective This study was designed to analyze the change in copy number in the DNA sequence in breast atypical ductal hyperplasia (ADH) of the breast, low- and high-grade ductal carcinoma in situ (LG-DCIS, HG-DCIS), as well as low- and high-grade invasive ductal carcinoma (LG-IDC, HG-IDC). This study was also designed to investigate the pathogenesis involved and provide a molecular cytogenetic mechanism.
Methods Comparative genomic hybridization (CGH) was used to detect the changes in the chromosomal copy number in 45 cases of ADH, LG-DCIS, HG-DCIS, LG-IDC, and HG-IDC.
Results The average gain and loss of the chromosome numbers were significantly lower in the low-grade cancer than in the high-grade cancer. However, there were no significant differences between carcinoma in situ and low-grade invasive carcinoma. The chromosomal abnormality in atypical hyperplasia was significantly higher than that in the cancerous tissue. The high-grade carcinoma in situ and the invasive cancer have the same chromosomal and locus cytogenetic abnormalities as those in many common deletions.
Conclusion Cytogenetic changes occur prior to the morphological changes in atypical hyperplasia, which may have close genetic ties with low-grade cancer and may be potential cancer precursor lesions. The identical grade cancers have collective molecular genetic alterations and com-path to progress.
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