Protein Expression of Livin in Triple-negative Breast Cancer and Its Value in Prognosis
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摘要:
目的 探讨凋亡抑制因子Livin在三阴性乳腺癌中的表达及与预后的关系, 分析三阴性乳腺癌的独立预后影响因素。 方法 用免疫组织化学SP法检测90例三阴性乳腺癌、35例癌旁乳腺组织、10例正常乳腺组织中Livin的表达; 结合临床病理特征和随访资料, 建立Cox模型进行回归分析。 结果 Livin在三阴性乳腺癌中的表达率为57.8%, 在癌旁乳腺组织中的表达率为34.3%, 在正常乳腺组织中表达率为0, 三者之间差异有统计学意义(P < 0.05)。Livin的表达与临床分期、脉管癌栓及腋淋巴结转移均有显著性差异(P < 0.05), 而与年龄、肿瘤大小、乳腺癌家族史及组织学分级无显著性差异(P > 0.05)。Kaplan-Meier生存曲线显示, Livin蛋白低表达组患者的无瘤生存时间及总生存时间明显优于Livin蛋白高表达组患者。多因素Cox回归分析显示, 年龄、临床分期、脉管癌栓及腋淋巴结转移情况是影响患者DFS的独立危险因素; 仅临床分期及腋淋巴结转移情况是影响患者总生存时间的独立危险因素; 而Livin均被剔除。 结论 Livin的异常高表达可能与三阴性乳腺癌的发生、发展及预后有相关性, 提示Livin的表达可能成为判断三阴性乳腺癌浸润进展及预后的指标之一。 Abstract:Objective To investigate the expression of the apoptosis inhibitor Livin and its correlations in the prognosis of triple-negative breast cancer (TNBC). Methods The expression of Livin was determined by immunohistochemical Streptavidin-Peroxidase method in 90 cases with TNBC, 35 with paraneoplastic tissues, and 10 with normal tissues. The Cox model analysis was estab- lished using clinico-pathological features and follow-up data. Results The positive expression of Livin was 57.8% in the TNBC tissues and 34.3 % in the paraneoplastic breast tissues. No Livin expression was observed in all 10 normal tissues, indicating the absence of statistical significance among the groups (P < 0.05). The expression of Livin was significantly correlated with clinical stage, lymph-vascu- lar invasion, and axillary lymph node metastasis (P < 0.05). The expression of Livin did not show correlations with the patient's age, tumor size, family history of breast cancer, and histologic grade (P > 0.05). The plotted survival curves by the Kaplan - Meier method dem- onstrated that disease-free survival and overall survival were significantly better in patients with low expression than in patients with high expression of Livin protein. Multivariate Cox regression analysis showed that age, clinical stage, lymph-vascular invasion, and ax- illary lymph node metastasis were independent risk factors affecting the five-year disease-free rate of the patients, whereas clinical stage and axillary lymph node metastasis were independent risk factors affecting the five-year overall rate of the patients. The Livin pro- tein was then rejected. Conclusion The abnormal expression of Livin is related to the development and prognosis of TNBC, suggesting that the expression of Livin may be used as one of the indicators in determining the progress and prognosis of TNBC. -
Key words:
- Triple-negative breast cancer /
- Livin /
- Immunohistochemistry /
- Prognosis /
- Multivariate analysis
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图 1 正常乳腺组织中Livin的阴性表达（SP×200）
Figure 1. Negative expression of Livin protein in normal tissues(SP×200)
图 2 乳腺癌组织中Livin的阳性表达（SP×400）
Figure 2. Positive expression of Livin protein in triple-negative breast cancer (TNBC)(SP×400)
图 3 Livin高表达组与低表达组DFS生存曲线的比较
Figure 3. Comparison of DFS between patients with low and high expressions of Livin
图 4 Livin高表达组与低表达组OS生存曲线的比较
Figure 4. Comparison of OS between patients with low and high expressions of Livin
表 1 Livin蛋白在三阴性乳腺癌中的表达与临床病理特征的关系 例
Table 1. Correlation between the expression of Livin protein and the pathological characteristics of TNBC
表 2 影响三阴性乳腺癌患者预后的单因素分析
Table 2. Univariate analysis of the prognosis of TNBC patients
表 3 影响三阴性乳腺癌患者DFS的多因素分析
Table 3. Multivariate Cox regression analysis of the disease-free survival (DFS) of TNBC patients
表 4 影响三阴性乳腺癌患者OS的多因素分析
Table 4. Multivariate Cox regression analysis of the overall survival (OS) of TNBC patients
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[1] Wang L, Zhang Q, Liu B, et al. Challenge and promise: roles for Livin in progression and therapy of cancer[J]. Mol Cancer Ther, 2008, 7(12): 3661-3669. doi: 10.1158/1535-7163.MCT-08-0480 [2] 吴国武, 曾娟紫. Livin蛋白在乳腺癌组织中的表达及意义[J]. 实用癌症杂志, 2011, 26(4): 372-373. https://www.cnki.com.cn/Article/CJFDTOTAL-SYAZ201104013.htm [3] 刘慧敏, 周士福, 时伟锋, 等. Livin和脆性组氨酸三联体的表达在确定乳腺癌保乳手术切缘及术中放疗安全靶区中的意义[J]. 肿瘤, 2010, 30(5): 419-422. https://www.cnki.com.cn/Article/CJFDTOTAL-ZZLL201005017.htm [4] Gazzaniga P, Gradilone A, Giuliani L, et al. Expression and prognostic significance of livin, survivin and other apoptosis-related genes in the progression of superficial bladder cancer[J]. Ann Oncol, 2003, 14(1): 85-90. doi: 10.1093/annonc/mdg002 [5] 周利军, 况建国. Livin蛋白在髓母细胞瘤中的表达及临床意义[J]. 江西医药, 2011, 46(2): 117-119. https://www.cnki.com.cn/Article/CJFDTOTAL-JXYY201102009.htm [6] Xi RC, Biao WS, Gang ZZ. Significant elevation of survivin and livin expression in human colorectal cancer: inversecorrelation between expression and overall survival[J]. Onkologie, 2011, 34(8-9): 428-432. [7] 菅金波, 葛莲英, 罗小玲, 等. Livin蛋白在人大肠癌组织中的表达及临床意义[J]. 中国癌症防治杂志, 2011, 3(1): 43-46. https://www.cnki.com.cn/Article/CJFDTOTAL-ZAZF201101015.htm [8] 陈晓燕, 韩子华. 胃癌组织中Livin蛋白的表达及其与临床病理特征和预后的关系[J]. 中国药物与临床, 2010, 10(11): 1230-1232. https://www.cnki.com.cn/Article/CJFDTOTAL-YWLC201011008.htm [9] Dai CH, Li J, Shi SB, et al. Survivin and Smac gene expressions but not livin are predictors of prognosis in non-small cell lung cancer patients treated with adjuvant chemotherapy following surgery[J]. Jpn J Clin Oncol, 2010, 40(4): 327-335. [10] Xiang Y, Yao H, Wang S, et al. Prognostic value of Survivin and Livin in nasopharyngeal carcinoma[J]. Laryngoscope, 2006, 116(1): 126-130. [11] Choi J, Hwang YK, Sung KW, et al. Expression of Livin, an antiapoptotic protein, is an independent favorable prognostic factor inchildhood acute lymphoblastic leukemia[J]. Blood, 2007, 109(2): 471-477. [12] 张丽, 赵晓辉, 佟仲生, 等. 356例三阴性乳腺癌的临床特征及预后多因素分析[J]. 中国肿瘤临床, 2010, 37(18): 1045-1049. doi: 10.3969/j.issn.1000-8179.2010.18.008 [13] 郭慧琳, 张幸平. 166例乳腺癌患者临床病理学特征及其与预后的关系[J]. 重庆医科大学学报, 2008, 33(4): 486-506. https://www.cnki.com.cn/Article/CJFDTOTAL-ZQYK200804027.htm -
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