Abstract:
Objective To study the effect of MLN4924 on the proliferation and apoptosis of lung epithelial carcinoma A549 cells.
Methods A549 cells were treated with MLN4924 at different concentrations and incubation times. The inhibition rate of cell growth was detected using the cell counting kit-8 (CCK-8) assay. Apoptosis was observed using Hoechst 33342 and flow cytometry. The protein expression of the associated proteins in cell apoptosis, including caspase-3, NF-κB p65, and CDT1, was determined through Western blot analysis.
Results The proliferation of A549 cells decreased when the concentration of MLN4924 increased or the incubation time was prolonged. MLN4924 induced S phase cell cycle arrest. The protein expression of the associated proteins caspase-3 and CDT1 was higher than that of the control group. NF-κB p65 was lower than that in the control group.
Conclusion MLN4924 effectively inhibited the proliferation and induced cell apoptosis in A549 cells. MLN4924 treatment induced the S phase cell cycle arrest, increased the caspase-3 protein expression, and inhibited the NF-κB pathway activation.