Abstract:
Objective This work aims to assess the anticancer mechanism of esophagus cancer vaccine for clinical application.
Methods Esophagus cancer cells were cultured, and the soluble antigens were extracted from them. Esophagus cancer vaccine was constructed with the antigen and superantigen SEC. Lymphocytes were isolated from peripheral blood, and then stimulated with the vaccine in vitro. Phenotypes of the cells were checked by FCM, and killing activity was tested with cytotoxic assay. Apoptosis of target cells were induced by effect cells checked with FCM.
Results The proliferation of the lymphocyte group stimulated by the vaccine is the strongest, which peaked at 72 h, thus raising CD8+T cell populations of CTLs. The killing activity of the lymphocyte group, which is stimulated by the vaccine against target cells is significantly higher than that of the lymphocyte group (P < 0.01). Apoptosis occurred in 33.44% of the target cells after induction of the effect cells and 6.38% in the control group. There is a significant difference between them (P < 0.01).
Conclusion The tumor vaccine constructed with esophagus cancer antigen and superantigen SEC can induce PBMC to activate and proliferate into CD8+CTL, with specific cytotoxicity against the cells from which the antigen comes from. The CTL induced by the vaccine can result in the apoptosis of tumor cells as target cells for the action of anticancer.