Effects of miRNA-375 on cell proliferation and apoptosis in the pancreatic cancer cell line Panc-1
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摘要:
目的 探讨miRNA-375的外源性表达对人胰腺癌细胞Panc-1增殖和凋亡的影响。 方法 将miRNA-375表达质粒或对照质粒转染Panc-1细胞, qRT-PCR方法检测miRNA-375在转染细胞中的表达水平, 细胞计数法、流式细胞术分别检测外源性miRNA-375表达后Panc-1细胞增殖、凋亡和周期的变化情况。 结果 miRNA-375表达质粒组与对照组比较, 其miRNA-375表达诱导性升高, 细胞增殖受到抑制, 细胞凋亡率增加, 细胞周期阻滞于G0~G1期。 结论 在胰腺癌细胞Panc-1中增加miRNA-375的表达, 可抑制细胞增殖, 促进细胞凋亡和细胞周期阻滞, miRNA-375在胰腺癌细胞Panc-1中可能发挥潜在的抑癌基因作用。 Abstract:Objective This study aimed to investigate the effect of the ectogenesis expression of miRNA-375 on cell proliferation and apoptosis in the human pancreatic cancer cell line Panc-1. Methods After transfection of the miRNA-375 vector, the expression levels of miRNA-375 in the Panc-1 cells were determined by quantitative real-time polymerase chain reaction.Cell counting was performed to evaluate the effects of miRNA-375 expression on cell proliferation.Flow cytometry was used to analyze apoptosis and the cell cycle. Results The expression levels of miRNA-375 were highly induced after miRNA-375 transfection.miRNA-375 restoration also inhibited cell proliferation, promoted cell apoptosis, and induced cell cycle arrest at G0-G1. Conclusion The abnormal expression of miRNA-375 in the pancreatic cancer cell line Panc-1 inhibits the canceration of cells by interfering with cellular biological behaviors, such as proliferation and apoptosis.miRNA-375 may act as a potential tumor suppressor in the pancreatic cancer cell Panc-1. -
Key words:
- pancreatic cancer /
- miRNA-375 /
- proliferation /
- apoptosis
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图 2 3组细胞中miRNA-375表达
Figure 2. Expressions of miRNA-375 in CON, miRNA-375-NC, and miRNA-375-mimics
图 3 miRNA-375对细胞增殖的影响
Figure 3. Effect of miRNA-375 on the regulation of cell proliferation
图 4 miRNA-375对Panc-1细胞凋亡的影响
Figure 4. Effect of miRNA-375 on the apoptosis of Panc-1 cells
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