检测肝癌微转移的研究新进展

吴飞翔; 杨琦; 黄盛鑫

doi:10.3969/j.issn.1000-8179.2013.02.014

检测肝癌微转移的研究新进展

doi: 10.3969/j.issn.1000-8179.2013.02.014

广西医科大学附属肿瘤医院肝胆外科（南宁市530021）

详细信息

通讯作者:
黄盛鑫 alexin.huang@gmail.com

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出版历程
- 收稿日期: 2012-09-28
- 修回日期: 2013-01-04

Research progress on micrometastasis of hepatocellular carcinoma

Department of Hepatobiliary Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021 China

More Information

Corresponding author: Shengxin HUANG; E-mail: alexin.huang@gmail.com

摘要

摘要: 原发性肝癌是全球高发病率的恶性肿瘤之一。手术切除是治疗原发性肝癌的首选手段, 然而手术切除后复发率、转移率高, 预后差是严重制约手术治疗远期疗效的重要因素。肝癌的转移是一个多步骤、多因素相互作用的复杂过程。近年来研究发现微转移是引起肿瘤复发和转移的根源, 微转移的检测对肿瘤的准确分期、指导治疗、判断预后有重要意义, 目前常用肝癌微转移灶检测标志物包括端粒酶、甲胎蛋白mRNA、MicroRNAs、上皮型钙黏素、CD44v6 mRNA和细胞角蛋白。本文从肝癌微转移灶的概念背景、标本来源、检测标志物、检测方法及相关临床意义等方面对肝癌微转移的研究进展进行综述, 旨在探讨微转移灶检测在肝癌临床治疗及预后中的重要意义。
- 原发性肝癌 /
- 微转移 /
- 标志物 /
- 诊断 /
- 治疗
Abstract: Primary liver cancer(PLC) is a malignant tumor with a very high incidence worldwide.Surgery is commonly the treatment of choice for PLC.However, tumor recurrence and metastasis after surgery seriously hamper the long-term efficacy of surgical treatment.The metastasis of hepatocellular carcinoma has long been known to involve a multi-step treatment process.Recent studies have shown that micrometastasis is the cause of tumor recurrence and metastasis.The detection of micrometastasis facilitates the accurate staging of the tumor, treatment, and prognosis.At this writing, markers for detecting liver cancer micrometastasis are available, including telomerase, al pha-fetoprotein mRNA, micro RNAs, E-cadherin, CD44v6mRNA, and cytokeratin.In this study, the researchers reviewed the research progress on the micrometastasis of liver cancer by examining the concept, source of specimen, detection methods, and clinical pathology to investigate the significance of each in the treatment and prognosis of the cancer.
- primary liver cancer /
- micrometastasis /
- markers /
- diagnosis /
- treatment

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参考文献(25)

[1]	Jemal A, Bray F, Center MM, et al. Global cancer statistics[J]. CACancer J Clin, 2011, 61(2): 69-90. doi: 10.3322/caac.20107
[2]	Wang JH, Wang CC, Hung CH, et al. Survival comparison between surgical resection and radiofrequency ablation for patients inBCLC very early/earlystage hepatocellular carcinoma[J]. J Hepatol, 2012, 56(2): 412-418. doi: 10.1016/j.jhep.2011.05.020
[3]	Huvos AG, Hutter RV, Berg JW. Significance of axillary macrometastases and micrometastases in mammary cancer[J]. Ann Surg, 1971, 173(1): 44-46. doi: 10.1097/00000658-197101000-00006
[4]	Kong SY, Park JW, Kim JO, et al. Alpha-fetoprotein and humantelomerase reverse transcriptase mRNA levels in peripheral bloodof patients withhepatocellular carcinoma[J]. J Cancer Res Clin Oncol, 2009, 135(8): 1091-1098. doi: 10.1007/s00432-009-0549-9
[5]	刘新兰, 陈萍, 魏建敏. CK20mRNA和hTERTmRNA表达与胃癌患者外周血微转移的研究[J]. 中国肿瘤临床, 2008, 35(22): 1286-1289. doi: 10.3969/j.issn.1000-8179.2008.22.007
[6]	Du XL, Ma QJ, Wu T, et al. Significance of alpha-fetoprotein mRNAlevel in hepatocellular carcinoma patients treated with radiofrequencyablation[J]. Hepatobiliary Pancreat Dis Int, 2007, 6(2): 172-175.
[7]	张利国, 周杰, 林建华. 外周血AFPmRNA、h-TERTmRNA检测对肝细胞肝癌患者预后的意义[J]. 山东医药, 2010, 50(16): 5-7. doi: 10.3969/j.issn.1002-266X.2010.16.003
[8]	Kitade Y, Akao Y. MicroRNAs and their therapeutic potential forhuman diseases: microRNAs, miR-143 and-145, function as anti-oncomirs and the application of chemically modified miR-143as an anti-cancer drug[J]. J Pharmacol Sci, 2010, 114(3): 276-280. doi: 10.1254/jphs.10R12FM
[9]	Murakami Y, Yasuda T, Saigo K, et al. Comprehensive analysis ofmicroRNA expression patterns in hepatocellular carcinoma andnon-tumoroustissues[J]. Oncogene, 2006, 25(17): 2537-2545. doi: 10.1038/sj.onc.1209283
[10]	Pineau P, Volinia S, McJunkin K, et al. iR-221 overexpression contributes to liver tumorigenesis[J]. Proc Natl Acad Sci USA, 2010, 107(1): 264-269. doi: 10.1073/pnas.0907904107
[11]	黄冠群, 孙建, 简志祥, 等. 利用芯片技术筛选肝癌早期复发密切相关的microRNA[J]. 实用医学杂志, 2011, 27(10): 1754-1756. doi: 10.3969/j.issn.1006-5725.2011.10.017
[12]	Evang JA, Berg JP, Casar-Borota O, et al. Reduced levels of E-cadherin correlate with progression of corticotroph pituitary tumours[J]. Clin Endocrinol(Oxf), 2011, 75(6): 811-818. doi: 10.1111/j.1365-2265.2011.04109.x
[13]	Saad AA, Awed NM, Abd Elkerim NN, et al. Prognostic significanceof E-cadherin expression and peripheral blood micrometastasis ingastric carcinomapatients[J]. Ann Surg Oncol, 2010, 17(11): 3059-3067. doi: 10.1245/s10434-010-1151-8
[14]	吴飞翔, 黄山, 杨春, 等. E-cadherin和β-catenin在肝细胞癌中的表达及意义[J]. 中国肿瘤, 2010, 19(6): 414-417. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHLU201006015.htm
[15]	石磊, 邵渊, 王作仁. 肝癌中ADAM10、EGFR和E-cadherin的表达分析及其与临床病理关系的研究[J]. 昆明医学院学报, 2011, 32(8): 23-29. https://www.cnki.com.cn/Article/CJFDTOTAL-KMYX201108007.htm
[16]	Rajarajan A, Stokes A, Bloor BK, et al. CD44 expression inoro-pharyngeal carcinoma tissues and cell lines[J]. PLoS One, 2012, 7(1): e28776. doi: 10.1371/journal.pone.0028776
[17]	Jang BI, Li Y, Graham DY, et al. The Role of CD44 in the Pathogenesis, Diagnosis, and Therapy of Gastric Cancer[J]. Gut Liver, 2011, 5(4): 397-405. doi: 10.5009/gnl.2011.5.4.397
[18]	刘黎黎, 符达, 朱玉珍, 等. CD90和CD44在人肝癌组织中的表达及临床意义[J]. 中国临床医学, 2012, 19(1): 11-13. doi: 10.3969/j.issn.1008-6358.2012.01.005
[19]	秦兴雷, 薛焕洲, 王作仁, 等. 肝外胆管癌淋巴结微转移的检测及其对预后的影响[J]. 中华医学杂志, 2010, 90(10): 678-682. doi: 10.3760/cma.j.issn.0376-2491.2010.10.010
[20]	Kelly KJ, Wong J, Gladdy R, et al. Prognostic impact ofRT-PCR-based detection of peritoneal micrometastases in patientswith pancreatic cancerundergoing curative resection[J]. Ann SurgOncol, 2009, 16(12): 3333-3339.
[21]	Lee SE, Jang JY, Kim MA, et al. Clinical implications of immunohistochemically demonstrated lymph node micrometastasis in resectablepancreatic cancer[J]. J Korean Med Sci, 2011, 26(7): 881-885. doi: 10.3346/jkms.2011.26.7.881
[22]	Guo J, Yao F, Lou Y, et al. Detecting carcinoma cells in peripheralblood of patients with hepatocellular carcinoma by immunomagneticbeads and rt-PCR[J]. J Clin Gastroenterol, 2007, 41(8): 783-788. doi: 10.1097/01.mcg.0000247996.19710.f2
[23]	赵挺, 胡福军, 凌志强. 原发性肝癌患者PBMC AFP mRNA表达与其微转移的相关性研究[J]. 中国误诊学杂志, 2002, 2(7): 976-979. doi: 10.3969/j.issn.1009-6647.2002.07.007
[24]	周学平, 杨广顺, 丛文铭, 等. 原发性肝癌瘤周肝组织内微转移的回顾性与前瞻性研究[J]. 中华肝胆外科杂志, 2005, 11(8): 510-514. doi: 10.3760/cma.j.issn.1007-8118.2005.08.002
[25]	Minata M, Nishida N, Komeda T, et al. Postoperative detection ofalpha-fetoprotein mRNA in blood as a predictor for metastatic recurrence ofhepatocellular carcinoma[J]. J Gastroenterol Hepatol, 2001, 16(4): 445-451. doi: 10.1046/j.1440-1746.2001.02461.x