IL-15对树突状细胞激活的T淋巴细胞免疫反应的作用观察

赵煜; 张涛; 谭勇; 彭晶晶; 周进军; 许涛; 付曾强

doi:10.3969/j.issn.1000-8179.2013.03.002

IL-15对树突状细胞激活的T淋巴细胞免疫反应的作用观察

Effect of IL-15 on T lymphocyte-mediated immune responses activated by dendritic cells

摘要

摘要:
目的观察IL-15和IL-2对树突状细胞疫苗(dendritic cells, DC)激活的淋巴细胞免疫反应的作用。
方法取健康小鼠的脾淋巴细胞, 在体外采用肿瘤抗原负载的DC疫苗与脾淋巴细胞混合后, 分别加入IL-15或IL-2培养7d。采用流式细胞术检测淋巴细胞免疫表型的变化; 使用酶联免疫斑点实验(ELISPOT)检测分泌干扰素(interferon, IFN)-γ的细胞数量; 利用51Cr释放法检测淋巴细胞对肿瘤细胞的杀伤活性。
结果 IL-15联合DC疫苗增强了T细胞反应, 包括淋巴细胞免疫表型和功能的改变。
结论与IL-2相比, IL-15能更好的增强DC疫苗诱发的免疫反应, 为IL-15与DC疫苗的联合应用提供了一定的依据。

Abstract:
Objective This study aimed to observe the ability of interleukin-15(IL-15) or interleukin-2(IL-2) to act as a potent adjuvant for a dendritic cell(DCs)–directed vaccine strategy to activate T-cell responses in vitro.
Methods Splenic lymphocytes from na ve mice were prepared.Then, bone marrow-derived DCs(BMDCs) were pulsed with tumor antigens and cultured with the splenocytes in the presence of IL-15 or IL-2 for 7 days.The activated T lymphocytes were examined by flow cytometric analysis, interferon-enzyme-linked immunospot, and cytotoxicity assays.
Results IL-15 resulted in an augmentation of T-cell responses following immunizations with BMDCs.The enhanced response was based on the phenotype characteristics of the lymphocytes and their functions.
Conclusion IL-15 may act as a potent adjuvant for the DC-directed vaccine strategy.

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