LBH589对人上皮性卵巢癌OVCAR-3细胞增殖的抑制作用及机制探讨

晁宏图; 邓君丽; 马一鸣; 王莉

doi:10.3969/j.issn.1000-8179.2013.03.003

LBH589对人上皮性卵巢癌OVCAR-3细胞增殖的抑制作用及机制探讨

Inhibitory function of LBH589 on the in vitro proliferation of human ovarian cancer cell lines and its mechanism

摘要

摘要:
目的探讨新型组蛋白去乙酰化酶抑制剂LBH589对人上皮性卵巢癌OVCAR-3细胞增殖抑制和促进凋亡作用及其机制。
方法不同浓度LBH589处理细胞后, 采用噻唑蓝(MTT)比色法检测对细胞增殖的影响; AO/EB(台盼蓝、吖啶橙溴化乙啶双染色法)检测细胞凋亡; Western blot检测聚腺苷二磷酸核糖聚合酶(PARP)、Caspase-3、Bcl-2、Bax蛋白水平。
结果 LBH589明显抑制OVCAR-3细胞增殖, 48 h半数抑制浓度(IC50)为0.12μM。Western blot检测发现Caspsae-3、PARP-85kD剪切蛋白增加, Bax表达增加, Bcl-2表达减少。
结论 LBH589在体外条件下能明显抑制卵巢癌细胞OVCAR-3细胞增殖, 诱导细胞凋亡。

Abstract:
Objective This study was designed to investigate the mechanism and effect of LBH589, a novel histone deacetylase inhibitor, on the growth and apoptosis of the human ovarian cancer cell line OVCAR-3.
Methods The cells were treated with LBH589 a different concentrations.Cell proliferation was determined using the methyl thiazol tetrazolium assay.The apoptotic rate of the cells was detected by AO/EB double-staining.Protein expressions of polyADP-ribose polymerase(PARP), caspase-3, bel-2, and bax were analyzed by western blot assay.
Results LBH589 significantly inhibited the proliferation of OVCAR-3 cells at a concentration of 0.12 μM/L based on a 48 h half-inhibitory concentration(IC50).Western blot assay showed that the expressions of cleaved PARP-85KD, caspase-3, and bax were increased, but bcl-2 expression was downregulated.
Conclusion LBH589 in vitro can significantly inhibit the proliferation and induce apoptosis of the human ovarian cancer cell line OVCAR-3.

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