Expression and significance of L1-CAM in invasive micropapillary carcinoma of the breast
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摘要:
目的 探讨L1细胞黏附分子(L1 cell adhesion molecules, L1-CAM)在乳腺浸润性微乳头状癌(Invasive micropapillarycarcinoma, IMPC)中的表达及其临床意义。 方法 应用免疫组织化学SP法, 检测97例乳腺IMPC中L1-CAM表达, 并以95例浸润性导管癌非特殊型(invasive ductal carcinoma, not otherwise specified, IDC-NOS)作为对照组。分析L1-CAM在IMPC的表达及与临床病理学特征和预后的关系。 结果 L1-CAM表达于肿瘤细胞膜及间质中的脉管内皮细胞, 在IMPC组主要表达于肿瘤细胞集团内细胞间的相互连接面。L1-CAM在IMPC中的表达率(50.5%)显著高于IDC-NOS组(18.9%), 并与IMPC的组织学分级、脉管侵犯、淋巴结转移、间质内脉管的L1-CAM表达及肿瘤细胞的P53表达呈正相关(P < 0.05), 与ER、PR表达呈负相关(P < 0.05)。单因素分析结果显示, IMPC患者中L1-CAM表达阳性患者预后不良。多因素分析结果显示IMPC中L1-CAM表达是影响患者术后生存的独立预后因素。 结论 L1-CAM可能在IMPC集团性生长、侵袭、转移中发挥重要作用, 可作为IMPC预后预测的标志物及靶向治疗研究的新靶点。 -
关键词:
- 乳腺浸润性微乳头状癌 /
- L1细胞粘附分子 /
- 淋巴结转移
Abstract:Objective The study aims to investigate the expression of L1 cell adhesion molecules(L1-CAM) in invasion micropapillary carcinoma(IMPC) and to analyze its clinical significance. Methods L1-CAM expression was analyzed in 97 IMPC cases and 95 invasive ductal carcinoma–not otherwise specified(IDC-NOS) cases via immunohistochemistry. The correlation of L1-CAM expression with clinicopathologic characteristics, which include the prognosis IMPC patients, was determined. Results The rate of L1-CAM expression in IMPC(50.5%) is significantly higher than that of IDC-NOS(18.9%). L1-CAM expression is also associated with histological grade, vessel invasion, lymph node metastasis, L1-CAM vessels, and protein p53 expression(P < 0.05), whereas it is negatively associated with ER and PR(P < 0.05). L1-CAM was predominantly expressed in the cell-cell interface of the cell clusters of IMPC and in the stroma vascular endothelial cells. Univariate analysis results indicate that expression of L1 is a risk factor that can predict the survival of IMPC patients(P < 0.05). Multivariate analyses results from a Cox's proportional hazards model show that a high expression of L1-CAM is an independent risk factor for the death of IMPC patients. Conclusion This study suggests that L1-CAM expression may have an important function in collective cell growth and is significantly associated with tumor invasion and metastasis. L1-CAM can be considered as biomarkers of prognosis and as a new target for IMPC therapy. -
图 1 L1-CAM在浸润性微乳头状癌及浸润性导管癌中的表达
Figure 1. L1-CAM expression in IMPC and IDC-NOS
A: Positive L1-CAM expression in IMPC(SP × 200). Strong membrane staining was mainly found in the interface of the cell clusters of IMPC; B: Positive L1-CAM expression in IMPC(SP×400); C: Negative L1-CAM expression in IMPC(SP×200); D: Positive L1-CAM expression in IDC-NOS (SP×400). Strong membrane staining was observed in tumor cells; E: Positive L1-CAM expression in the endothelial cells of IMPC(SP×400); F: Negative L1-CAM expression in IDC-NOS(SP×400)
表 1 L1-CAM表达和乳腺IMPC临床病理学特征的关系
Table 1. Correlations between L1-CAM expression and clinicopathologic features in IMPC cases
表 2 97例乳腺IMPC患者预后的临床变量多因素分析
Table 2. Multivariate analysis of clinicopathologic variables as prognostic factors for overall survival in 97 IMPC cases
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