L1-CAM在乳腺浸润性微乳头状癌的表达及其意义

张超; 范宇; 李伟东; 李崖青; 李帅; 钱晓龙; 林阳; 刘芳芳; 郎荣刚; 谷峰; 付丽

doi:10.3969/j.issn.1000-8179.2013.04.005

L1-CAM在乳腺浸润性微乳头状癌的表达及其意义

doi: 10.3969/j.issn.1000-8179.2013.04.005

天津医科大学附属肿瘤医院乳腺病理研究室，乳腺癌防治教育部重点实验室，天津市肿瘤防治重点实验室（天津市300060）

基金项目:

国家自然科学基金项目 30930038

教育部长江学者乳腺癌创新团队奖励计划项目 IRT 0743

详细信息

通讯作者:
付丽 fulijyb@hotmail.com

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出版历程
- 收稿日期: 2012-11-27
- 修回日期: 2013-01-15

Expression and significance of L1-CAM in invasive micropapillary carcinoma of the breast

Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Breast Cancer Prevention and Treatment, Ministry of Education, Key Laboratory of Cancer Prevention and Treatment of Tianjin City, Tianjin 300060, China

Funds:

the National Natural Science Foundation of China 30930038

the Program for Changjiang Scholars Innovative Research Team of Breast Cancer in University IRT 0743

More Information

Corresponding author: Li FU; E-mail: fulijyb@hotmail.com

摘要

摘要: 目的探讨L1细胞黏附分子(L1 cell adhesion molecules, L1-CAM)在乳腺浸润性微乳头状癌(Invasive micropapillarycarcinoma, IMPC)中的表达及其临床意义。方法应用免疫组织化学SP法, 检测97例乳腺IMPC中L1-CAM表达, 并以95例浸润性导管癌非特殊型(invasive ductal carcinoma, not otherwise specified, IDC-NOS)作为对照组。分析L1-CAM在IMPC的表达及与临床病理学特征和预后的关系。结果 L1-CAM表达于肿瘤细胞膜及间质中的脉管内皮细胞, 在IMPC组主要表达于肿瘤细胞集团内细胞间的相互连接面。L1-CAM在IMPC中的表达率(50.5%)显著高于IDC-NOS组(18.9%), 并与IMPC的组织学分级、脉管侵犯、淋巴结转移、间质内脉管的L1-CAM表达及肿瘤细胞的P53表达呈正相关(P < 0.05), 与ER、PR表达呈负相关(P < 0.05)。单因素分析结果显示, IMPC患者中L1-CAM表达阳性患者预后不良。多因素分析结果显示IMPC中L1-CAM表达是影响患者术后生存的独立预后因素。结论 L1-CAM可能在IMPC集团性生长、侵袭、转移中发挥重要作用, 可作为IMPC预后预测的标志物及靶向治疗研究的新靶点。
- 乳腺浸润性微乳头状癌 /
- L1细胞粘附分子 /
- 淋巴结转移
Abstract: Objective The study aims to investigate the expression of L1 cell adhesion molecules(L1-CAM) in invasion micropapillary carcinoma(IMPC) and to analyze its clinical significance. Methods L1-CAM expression was analyzed in 97 IMPC cases and 95 invasive ductal carcinoma–not otherwise specified(IDC-NOS) cases via immunohistochemistry. The correlation of L1-CAM expression with clinicopathologic characteristics, which include the prognosis IMPC patients, was determined. Results The rate of L1-CAM expression in IMPC(50.5%) is significantly higher than that of IDC-NOS(18.9%). L1-CAM expression is also associated with histological grade, vessel invasion, lymph node metastasis, L1-CAM vessels, and protein p53 expression(P < 0.05), whereas it is negatively associated with ER and PR(P < 0.05). L1-CAM was predominantly expressed in the cell-cell interface of the cell clusters of IMPC and in the stroma vascular endothelial cells. Univariate analysis results indicate that expression of L1 is a risk factor that can predict the survival of IMPC patients(P < 0.05). Multivariate analyses results from a Cox's proportional hazards model show that a high expression of L1-CAM is an independent risk factor for the death of IMPC patients. Conclusion This study suggests that L1-CAM expression may have an important function in collective cell growth and is significantly associated with tumor invasion and metastasis. L1-CAM can be considered as biomarkers of prognosis and as a new target for IMPC therapy.
- invasive micropapillary carcinoma of the breast /
- L1 cell adhesion molecules /
- lymph node metastasis

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图 1 L1-CAM在浸润性微乳头状癌及浸润性导管癌中的表达

Figure 1. L1-CAM expression in IMPC and IDC-NOS

A: Positive L1-CAM expression in IMPC(SP × 200). Strong membrane staining was mainly found in the interface of the cell clusters of IMPC; B: Positive L1-CAM expression in IMPC(SP×400); C: Negative L1-CAM expression in IMPC(SP×200); D: Positive L1-CAM expression in IDC-NOS (SP×400). Strong membrane staining was observed in tumor cells; E: Positive L1-CAM expression in the endothelial cells of IMPC(SP×400); F: Negative L1-CAM expression in IDC-NOS(SP×400)

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表 1 L1-CAM表达和乳腺IMPC临床病理学特征的关系

Table 1. Correlations between L1-CAM expression and clinicopathologic features in IMPC cases

表 2 97例乳腺IMPC患者预后的临床变量多因素分析

Table 2. Multivariate analysis of clinicopathologic variables as prognostic factors for overall survival in 97 IMPC cases

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