Clinical significance of h-TERT and c-Met expression in the peripheral blood circulation of pancreatic cancer patients
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摘要:
目的 探讨胰腺癌患者外周血循环肝细胞生长因子受体c-Met和端粒酶亚催化单位(human telomerase reverse transcriptase, h-TERT)表达的临床意义。 方法 联合应用纳米免疫磁珠及巢式PCR方法检测胰腺癌患者外周血循环中c-Met和h-TERT的表达以确定微转移是否存在, 并探讨其临床意义。 结果 胰腺癌患者外周血循环c-Met与h-TERT的表达与性别、年龄、肿瘤大小、血清CA-199及CEA无统计学相关(P > 0.05)。不同肿瘤分期间c-Met和h-TERT阳性表达率有显著性差异(P < 0.05), 外周血c-Met与h-TERT表达阳性患者有肿瘤复发风险, h-TERT阳性患者较c-Met阳性患者肿瘤复发率高(P < 0.05)。c-Met表达阳性的患者无瘤生存中位时间为12个月, 阴性患者为20个月(P=0.044);h-TERT表达阳性患者无瘤生存中位时间为9个月, 阴性患者为15个月(P < 0.001)。 结论 外周血存在微转移的胰腺癌患者肿瘤复发率高, 外周血h-TERT较c-Met表达阳性患者肿瘤复发率高, 预后更差。 Abstract:Objective This study aimed to investigate the clinical significance of the human telomerase reverse transcriptase(h-TERT) and c-Met gene expressions in the peripheral blood of pancreatic cancer patients. Methods Magnetic cell sorting and reverse transcription-nest-polymerase chain reaction were performed to detect h-TERT and c-Met expressions in the peripheral blood of pancreatic cancer and determine whether or not micrometastasis was present. The clinical significance of micrometastasis was analyzed. Results The positive rates of c-Met and h-TERT did not significantly correlate with gender, age, cancer size, cancer antigen 19-9, and carcinoembryonic antigen in the serum(P > 0.05). The positive rates of c-Met and h-TERT genes in the peripheral blood of pancreatic cancer patients significantly correlated with TNM staging(P < 0.05). The recurrence rate was higher in pancreatic cancer patients with positive h-TERT compared with that in patients with positive c-Met. The median disease-free survival time for patients with positive h-TERT and c-Met was 12 and 9 months, respectively. The median disease-free survival time was 20 months(P=0.044) for patients with negative h-TERT. For pancreatic cancer patients with negative c-Met, the median disease-free survival time was 15 months(P < 0.001). Conclusion The prognosis is poorer in h-TERT-positive patients than in c-Met-positive patients. The relapse rate was higher in h-TERT-positive patients who have poor prognosis. -
Key words:
- pancreatic cancer /
- micrometastasis /
- c-Met /
- h-TERT
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表 1 引物序列及产物扩增长度
Table 1. Primer sequences and amplification primer length
表 2 胰腺癌患者外周血循环c-Met和h-TERT基因的表达与临床参数的关系
Table 2. Relationship of the expression of h-TERT and c-Met genes in the peripheral blood circulation with the clinical parameters
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