Rac1 VEGF在肝细胞癌中的表达和肿瘤血管形成关系

孙旭日; 李新丰; 王伟; 刘玉琪

doi:10.3969/j.issn.1000-8179.2013.04.008

Rac1 VEGF在肝细胞癌中的表达和肿瘤血管形成关系

doi: 10.3969/j.issn.1000-8179.2013.04.008

孙旭日^①, ,,
李新丰^②,
王伟^②,
刘玉琪^①

①.
福建医科大学附属第二医院重症医学科（福建省泉州市362000）
②.
普外科

基金项目:

福建省卫生厅青年科研课题 2007-02-18

福建省泉州市技术研究与开发项目计划重点项目 2009Z18

详细信息

通讯作者:
孙旭日 glorysunxuri@yahoo.com.cn

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出版历程
- 收稿日期: 2012-06-26
- 修回日期: 2012-12-07

Rac1 and VEGF expressions in hepatocellular carcinoma and its relationship with tumor angiogenesis

Xuri SUN^{①
, ,},
Xinfeng LI^②,
Wei WANG^②,
Yuqi LIU^①

①.
Intensive Care Unit, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000 China
②.
Department of General Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000 China

Funds:

the Foundation of Youth Research Program of the Department of Public Health, Fujian Province 2007-02-18

the Key Project of the Technology Research and Development Program, Quanzhou, Fujian Province 2009Z18

More Information

Corresponding author: Xuri SUN; E-mail: glorysunxuri@yahoo.com.cn

摘要

摘要: 目的探讨Rac1、VEGF蛋白在肝细胞癌组织的表达及其与肿瘤血管形成的关系。方法应用免疫组织化学法检测Rac1、VEGF在43例肝细胞癌组织、43例癌旁肝组织及21例正常肝组织的表达, 采用图像分析软件测定Rac1蛋白、VEGF蛋白的平均光密度, 根据CD34染色的血管内皮细胞计数肿瘤MVD。结果 Rac1蛋白在癌组织、癌旁肝组织及正常肝组织均有表达, 位于胞浆, 癌组织表达高于癌旁肝组织(P < 0.01)、正常肝组织(P < 0.01);VEGF蛋白在癌组织、癌旁肝组织及正常肝组织均有表达, 位于胞浆, 癌组织高于癌旁肝组织(P < 0.01)、正常肝组织(P < 0.01);Rac1、VEGF与MVD之间均呈正相关(r=0.490, P=0.001;r=0.355, P=0.019), Rac1与VEGF之间呈正相关(r=0.583, P < 0.001)。结论 Rac1可能通过上调VEGF的表达促进肿瘤血管生成, 参与HCC的侵袭、转移机制。
- 肝细胞癌 /
- Rac1 /
- VEGF /
- 微血管密度 /
- 免疫组织化学
Abstract: Objective This study aimed to detect Rac1 and vascular endothelial growth factor(VEGF) expressions in hepatocellular carcinoma(HCC) and to correlate this expression with tumor angiogenesis. Methods Rac1 and VEGF expressions in 43 specimens of HCC, 43 specimens of adjacent nontumorous liver tissue, and 21 specimens of normal liver tissues were detected by immunohistochemistry. Image analysis software was used to conduct a quantitative analysis of Racl and VEGF expressions by accessing their mean optical density(MOD). Microvascular density(MVD) was determined by labeling the vascular endothelial cells with CD34. Results Rac1 protein expression occurred mainly in the HCC tissue of the cytoplasm. MOD in HCC tissue was markedly higher than those in adjacent nontumorous liver tissue(P < 0.01) and in normal liver tissue(P < 0.01). The expression of VEGF protein was higher in HCC tissue than those in adjacent non-tumorous liver tissue(P < 0.01) and normal liver tissue(P < 0.01). Moreover, the Rac1 and VEGF protein expressions in HCC were positively correlated with MVD(r=0.490, P=0.001;r=0.355, P=0.019). Rac1 protein expression in HCC tissue was positively correlated with the VEGF protein expression in HCC tissue(r=0.583, P=0.000). Conclusion Rac1 possibly promotes tumor angiogenesis through the upregulation of VEGF expression, and is associated with HCC invasion and metastasis.
- hepatocellular carcinoma /
- Rac1 /
- VEGF /
- MVD /
- immunohistochemistry

HTML全文

图 1 Rac1蛋白在HCC癌组织的表达（免疫组化×400）

Figure 1. Rac1 protein expression in HCC tissue(IHC×400)

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图 2 VEGF蛋白在HCC癌组织的表达（免疫组化×400）

Figure 2. VEGF protein expression in HCC tissue(IHC×400)

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图 3 CD34在HCC癌组织的表达（免疫组化×100）

Figure 3. CD34 expression in HCC tissue(IHC×100)

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表 1 Rac1、VEGF蛋白表达及MVD与HCC不同临床病理指标之间的关系

Table 1. Correlation of the protein expression of Rac1, VEGF and MVD with clinicopathologic parameters of HCC

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