Abstract:
Objective This work studied the interaction of secretory immunoglobulin A(SIgA), interleukin 6(IL-6), and dendritic cells(DCs) in oral cancer.
Methods Saliva samples from each oral cancer patient were collected before surgery to detect the SIgA and IL-6 levels.Enzyme-linked immunosorbent assay and flow cytometry were used to determine the expression of CD1a, CD83, CD80, and CD86 on DCs in cancer tissues.Normal oral epithelial tissues were confirmed by pathology and used as the controls.
Results The SIgA content was lower in the saliva of oral cancer patients than in those of the normal individuals.By contrast, the IL-6 content was evidently higher in the saliva of cancer patients than in that of the healthy controls.SIgA and IL-6 were negatively correlated with each other(r=-0.993, P < 0.05).The expression levels of CD1a, CD83, CD80, and CD86 in cancer tissues were lower than those in normal tissues(P < 0.05).The CD80 and CD86 expression levels in the cancer tissues were not associated with the histological grading and pathological types(P > 0.05).Correlation analysis showed that the CD80 and CD86 levels were negatively correlated with the clinical stage and lymph node metastases(P < 0.05).
Conclusion The SIgA and IL-6 content can be used as auxiliary indicators for oral cancer diagnosis.The increasing IL-6 level could account for the decreased SIgA production.Immune deficiency occurs in the DCs of oral cancer patients, and the expression levels of CD80 and CD86 reflect this prognostic evaluation.IL-6 may inhibit the formation of SIgA and cause the immune tolerance of DC.Thus, the effective immune response is lost, thereby promoting carcinogenesis and the progress of oral cancer.
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