STAT3与miRNA-21在舌鳞状细胞癌中异常表达的相关性研究

李莎莎; 周旋; 张强; 张仑

doi:10.3969/j.issn.1000-8179.2013.06.006

STAT3与miRNA-21在舌鳞状细胞癌中异常表达的相关性研究

Abnormal expression of STAT3 and miRNA-21 in oral squamous cell carcinoma

摘要

摘要:
目的探讨不同分化程度人舌鳞状细胞癌组织及细胞系中STAT3及miRNA-21表达的相关性及其和预后的关系。
方法 Western Blot法测定舌鳞癌细胞中STAT3表达, 免疫组织化学染色法检测舌鳞癌组织标本中STAT3蛋白表达水平, 采用荧光实时定量PCR法检测舌鳞癌细胞中miRNA-21的表达, 采用荧光原位杂交法测定舌鳞癌标本中miRNA-21表达, Spearman等级相关分析STAT3、miRNA-21表达在舌鳞癌中表达的相关性, Kaplan-Meier法分析STAT3、miRNA-21不同表达状态与生存期的关系。
结果 STAT3和miRNA-21在舌鳞癌细胞及组织标本中过表达; 舌鳞癌标本中STAT3、miRNA-21随肿瘤分化程度越差, 其表达越高; STAT3和miRNA-21表达呈正相关(r_s=0.574, P < 0.001);STAT3、miRNA-21与生存期为负相关, STAT3和miRNA-21高表达患者的预后较低表达者差。
结论 STAT3、miRNA-21的表达状态对预测舌鳞癌患者的预后具有一定的价值。 < /span >

Abstract:
Objective This study aims to(i) detect the expression of both microRNA-21(miR-21) and signal transducer and activator of transcription 3(STAT3) in oral squamous cell carcinoma(OSCC) tissue samples and cell lines, and(ii) determine the correlation between their expression and prognosis.
Methods miR-21 expression in OSCC cell lines and tissue samples was measured by real-time polymerase chain reaction and in situ hybridization, respectively.Western blot analysis and immunohistochemical staining were used to determine the STAT3 expression in the OSCC cell lines and tissue samples.The correlations between the miR-21/STAT3 expression and the pathological grade were analyzed using the Spearman rank correlation.
Results Abnormal activations of miR-21 and STAT3 were observed in the OSCC cell lines and the tissue samples.miR-21(r_s=0.879, P=0.000) and STAT3(r_s=0.837, P=0.000) were positively correlated with the tumor stage classification.Survival rate was higher in the group with miR-21 overexpression than in the group with low expression.
Conclusion miR-21 and STAT3 are potential predictors for the prognosis of OSCC patients.The assessment of miR-21 and STAT3 expression can help determine a novel pathway for individualized targeted therapy.

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