Wnt家族成员在TA2小鼠乳腺癌生成过程中基因表达变化规律

刘芳; 孙保存; 张丹芳; 王进京; 董学易; 古强; 赵秀兰

doi:10.3969/j.issn.1000-8179.2013.07.001

Wnt家族成员在TA2小鼠乳腺癌生成过程中基因表达变化规律

Dynamic Wnt gene expression in the oncogenesis of breast cancer in Tianjin albino 2 strain mice

摘要

摘要:
目的研究乳腺癌生成过程中, Wnt家族成员表达的变化规律。
方法收集自发乳腺癌小鼠42只, 记录其详细资料。采用基因芯片筛选正常TA2小鼠乳腺组织和自发乳腺癌TA2小鼠乳腺癌组织中的差异表达基因, 随后用Real-time PCR进行验证, 并检测TA2小鼠乳腺癌形成过程中相关基因表达的变化。
结果 TA2自发乳腺癌小鼠平均见瘤年龄为(336.706±85.05)d, 平均分娩次数为(3.767±1.79)次。分娩次数最多者为7次, 其平均见瘤年龄只有251.5 d。TA2小鼠基因芯片结果发现Wnt家族成员中, Wnt1、Wnt10b、Wnt5a、Wnt5b在正常乳腺组织和乳腺癌组织中表达具有差异。Real time PCR的结果证实了以上结果, 并发现Wnt1、Wnt10b、Wnt5a在癌前病变和乳腺癌组织中表达高于正常乳腺组织, 而Wnt5b在乳腺癌组织中表达低于正常乳腺组织。
结论 Wnt1、Wnt5a、Wmt10b在TA2小鼠乳腺癌生成过程中起促进作用, 而Wnt5b的作用可能是抑制小鼠乳腺癌的生成。

Abstract:
Objective This work aims to investigate the dynamic gene expression of Wnt family members in the oncogenesis of breast cancer in Tianjin albino 2 strain(TA2) mice.
Methods Clinical specimens of spontaneous breast cancers were harvested from 42 female TA2 mice, and mammary glands were collected from tissues of normal and pregnant TA2 mice.A gene chip was used to identify the differentially expressed genes between the tissues of breast cancer and the normal mammary gland.They were subsequently validated by real-time polymerase chain reaction and the change in the Wnt expressions in the oncogenesis of breast cancer in TA2 mice.
Results The average age of the TA2 mice at carcinogenesis was 336.706 ± 85.05 d, and the average frequency of delivery was 3.767 ± 1.79.Compared with the mammary gland of the normal TA2 mice, Wnt1, Wnt5a, and Wnt10b were obviously up-regulated in the breast cancer tissue of the TA2 mice.However, the expression of Wnt5b was down-regulated in the normal mammary gland and the breast cancer tissues of the TA2 mice.
Conclusion Wnt1, Wnt5a, and Wnt10b may promote the progression of breast cancer, whereas Wnt5b may inhibit the development of TA2 breast cancer.

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