冯慧, 田菁, 孙和熙, 张山岭, 鞠宝辉, 郝权. 卵巢癌腹腔微环境中肿瘤相关巨噬细胞表型特点及其TLR表达谱的研究[J]. 中国肿瘤临床, 2013, 40(7): 375-379. DOI: 10.3969/j.issn.1000-8179.2013.07.002
引用本文: 冯慧, 田菁, 孙和熙, 张山岭, 鞠宝辉, 郝权. 卵巢癌腹腔微环境中肿瘤相关巨噬细胞表型特点及其TLR表达谱的研究[J]. 中国肿瘤临床, 2013, 40(7): 375-379. DOI: 10.3969/j.issn.1000-8179.2013.07.002
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Hui FENG, Jing TIAN, Hexi SUN, Shanling ZHANG, Baohui JU, Quan HAO. Mixed-phenotype of tumor-associated macrophages in the peritoneal environment of ovarian cancer and its TLR expression characteristics[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(7): 375-379. DOI: 10.3969/j.issn.1000-8179.2013.07.002
Citation: Hui FENG, Jing TIAN, Hexi SUN, Shanling ZHANG, Baohui JU, Quan HAO. Mixed-phenotype of tumor-associated macrophages in the peritoneal environment of ovarian cancer and its TLR expression characteristics[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(7): 375-379. DOI: 10.3969/j.issn.1000-8179.2013.07.002
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卵巢癌腹腔微环境中肿瘤相关巨噬细胞表型特点及其TLR表达谱的研究

Mixed-phenotype of tumor-associated macrophages in the peritoneal environment of ovarian cancer and its TLR expression characteristics

    摘要
  • 摘要:
      目的  探讨卵巢癌腹水微环境中肿瘤相关巨噬细胞(TAM)浸润情况及其表型特征和TAM的Tol样受体表达情况。
      方法  应用免疫组织化学方法及流式细胞术检测卵巢癌及妇科良性病变腹水中CD68+TAM及其亚型的比例情况。应用Real-timePCR检测不同亚型TAM的Tol样受体的表达情况。
      结果  23例卵巢癌腹水细胞CD68+TAM平均值为73.6个/HP, 20例良性病变平均计数为50.2个/HP。两组间CD68+TAM浸润差异无统计学意义(P > 0.05)。CD68+TAM在卵巢癌及对照组腹水中所占比例无差异, 分别为31.7%和18.8%(P > 0.05)。卵巢癌腹水中M2型TAM占全部巨噬细胞54.3%, 明显高于对照组10.0%(P < 0.05);对照组M1型TAM占优势, 为53.3%, 明显高于卵巢癌组的30.5%(P < 0.01)。不同亚型巨噬细胞Toll样受体表达差异无统计学意义。
      结论  卵巢癌腹腔微环境中同时存在M1和M2型TAM, 但M2型TAM在卵巢癌中占优势, 而M1型TAM在良性病变中占优势。Toll样受体不能区分M1-TAM与M2-TAM。

     

    Abstract:
      Objective  This study investigates the following: proportions of tumor-associated macrophage(TAM) and its subpopulations in the ascite of ovarian cancer(OC) patients and expression of mammalian homologs of the toll-like receptors(TLRs) in different TAM subpopulations.
      Methods  CD68+TAM expression was detected by streptavidin-peroxidase immunohistochemical staining.Proportions of TAM and its subpopulations were identified by flow cytometry.RNA assessment of TLR was performed by real-time polymerase chain reaction.
      Results  Mean density of CD68+TAM was similar between OC patients and those with benign tumor(73.6 vs.50.2 per vision field, P > 0.05).CD68+ TAM in the ascite exhibited no difference between OC patients and those with benign tumor(P > 0.05).However, M1 macrophage in OC ascites was significantly less than those found in benign tumor(30.5% vs.53.3%;P < 0.01).M2 macrophage in OC ascites was significantly higher than those found in benign tumor(54.3% vs.10.1%;P < 0.05).TLR level was similar in M1 and M2 macrophages(P > 0.05).
      Conclusion  A mixed-phenotype of tumor-associated macrophages was found in the peritoneal environment.M2 macrophages were dominant in the ascite of OC, whereas M1 macrophages were predominant in the benign tumor.TLRs were not the key factors that affected the polarization of macrophages.

     

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