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摘要:
目的 研究EHD2在前列腺癌中的表达以及EHD2表达水平对前列腺癌细胞系增殖与转化能力的影响。 方法 免疫组织化学方法检测EHD2在14例前列腺癌组织及7例前列腺增生组织中的表达。用EHD2干扰表达质粒、过表达质粒和对照载体分别转染293T细胞, 制备慢病毒, 感染DU145细胞, 建立稳定细胞系。用Western blot技术检测EHD2表达情况。采用细胞计数法、二维克隆形成实验、Soft Agar实验分别检测EHD2沉默或过表达对前列腺癌细胞增殖能力、克隆形成能力和转化特性的影响。 结果 免疫组织化学显示EHD2在前列腺癌组织中表达量明显低于前列腺增生组织。EHD2干扰表达后DU145细胞的增殖能力、二维克隆形成能力、三维克隆形成能力均明显增强(P < 0.01);EHD2过表达后DU145细胞的增殖能力和三维克隆形成能力均减弱(P < 0.01和P < 0.05)。 结论 EHD2的表达水平对DU145前列腺癌细胞的增殖与转化特性具有明显的调节作用, EHD2可能是前列腺癌的新抑癌基因。 Abstract:Objectives This research examined EHD2 expression level in prostate cancer and identified its effects on proliferation and transformation of prostate cancer cells. Methods Immunohistochemistry was used to detect EHD2 expression level in prostate cancer tissue and benign prostatic hyperplasia.The lentiviral system facilitates EHD2 overexpression or silencing in DU145 cells.Cell counting, colony formation, and soft agar experiments were used to determine cell proliferation and transformation characteristics. Results EHD2 expression decreased in cancer cells compared with cells in hyperplasia tissue.EHD2 downregulation promoted DU145 cell growth and colony formation(P < 0.01).Opposite effects were observed with EHD2 overexpression. Conclusion EHD2 expression was suppressed in prostate tumor cells.Therefore, EHD2 may be a novel tumor suppressor in prostate cancer. -
Key words:
- prostate cancer /
- EHD2 /
- proliferation
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图 1 EHD2在前列腺癌组织和前列腺增生组织中的IHC染色典型表现(DAB×400)
Figure 1. Representative immunohistochemistry images of EHD2 stain-ing in prostate cancer tissue and benign prostatic hyperplasia DAB×400
A: Benign prostatic hyperplasia tissue; B: Prostate cancer tissue
图 2 免疫印迹检测在DU145细胞中EHD2干扰和过表达效果
Figure 2. Western blot analysis of the silencing and overexpression ef-fects of EHD2 in DU145 cells
图 3 EHD2的表达水平对DU145细胞增殖能力的影响
Figure 3. Effects of EHD2 expression on DU145 proliferation
A: Silencing group of EHD2;B: Over-expression group of EHD2
图 4 EHD2干扰对DU145细胞二维克隆形成能力的影响
Figure 4. Effects of EHD2 silencing on DU145 colony formation
图 5 EHD2表达水平对DU145细胞三维克隆形成能力的影响
Figure 5. Effects of EHD2 expression on DU145 colony formation in soft agar
A: Compared with the control group, DU145 colony formation was strengthened with EHD2 silencing; B: Compared with the control group, DU145 colony formation was weakened with EHD2 overexpression
表 1 EHD2在前列腺癌组织和前列腺增生组织中的表达情况 例（%）
Table 1. EHD2 expression level in prostate cancer tissue and benign prostatic hyperplasia Case(%)
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