Abstract:
Objective Compared with invasive ductal carcinoma-not otherwise specified(IDC-NOS), invasive micropapillary carcinoma(IMPC) shows more distinctive morphologic characteristics and a biological feature with high incidence of axillary lymph node metastases.This study used comparative proteomics technology to find the differentially expressed proteins between IMPC and IDC-NOS, followed by verification.Afterward, the mechanisms of the special pathological morphology and biological behavior of IMPC was explored from the point view of proteomics.
Methods Candidate molecules were obtained by examining the differential expression spots in the frozen tissues of one IMPC and one IDC-NOS.Two-dimensional polyacrylamide gel electrophoresis and mass spectrum were used as tools for analysis.The immunohistochemical stain assay was used to verify the expression of these candidate molecules in the tissue sections of 43 IMPC and 30 IDC-NOS frozen samples.
Results One differentially expressed protein, namely, Heat shock protein 27(Hsp27), was screened out.The expression of Hsp27 was up-regulated in IMPC compared with that in IDC-NOS.Hsp27 was mainly expressed in the plasma of tumor cells, and the expression was significantly higher in IMPC than in IDC-NOS(Z=-3.236, P=0.001).The expression of Hsp27 positively correlated with the estrogen receptor(r=0.319, P=0.037) and metastasis in lymph nodes(r=0.444, P=0.003).No correlation was found between Hsp27 and the patient's age, pathologic stage, and progesterone receptor or human epidermal growth factor receptor 2.
Conclusion Hsp27 shows over-expression in IMPC and is positively correlated with metastasis in lymph nodes.Hsp27 may play an important role in the formation of the special pathological morphology of IMPC, as well as the high rate of lymph node metastasis.
下载: