姜孝芳, 李卉, 刘玲, 王洪江, 李秀梅, 陈艳, 庞作良, 谌宏鸣, 尹娜, 李艳, 沙亚哈提·别尔克哈之, 李惠武. 哈萨克族食管癌患者中Lrig1通过MEK-ERK信号通路调控NF-κB及MMP-2表达[J]. 中国肿瘤临床, 2013, 40(10): 575-578,582. DOI: 10.3969/j.issn.1000-8179.2013.10.006
引用本文: 姜孝芳, 李卉, 刘玲, 王洪江, 李秀梅, 陈艳, 庞作良, 谌宏鸣, 尹娜, 李艳, 沙亚哈提·别尔克哈之, 李惠武. 哈萨克族食管癌患者中Lrig1通过MEK-ERK信号通路调控NF-κB及MMP-2表达[J]. 中国肿瘤临床, 2013, 40(10): 575-578,582. DOI: 10.3969/j.issn.1000-8179.2013.10.006
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Xiaofang JIANG, Hui LI, Ling LIU, Hongjiang WANG, Xiumei LI, Yan CHEN, Zuoliang PANG, Hongming CHEN, Na YIN, LI Yan, BIE Erkehazhi·SHAYahati, Huiwu LI. NF-κB and MMP-2 expression regulated by Lrig1 through the MEK-ERK signaling pathway in Hazaks' esophageal squamous cell carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(10): 575-578,582. DOI: 10.3969/j.issn.1000-8179.2013.10.006
Citation: Xiaofang JIANG, Hui LI, Ling LIU, Hongjiang WANG, Xiumei LI, Yan CHEN, Zuoliang PANG, Hongming CHEN, Na YIN, LI Yan, BIE Erkehazhi·SHAYahati, Huiwu LI. NF-κB and MMP-2 expression regulated by Lrig1 through the MEK-ERK signaling pathway in Hazaks' esophageal squamous cell carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(10): 575-578,582. DOI: 10.3969/j.issn.1000-8179.2013.10.006
shu

哈萨克族食管癌患者中Lrig1通过MEK-ERK信号通路调控NF-κB及MMP-2表达

NF-κB and MMP-2 expression regulated by Lrig1 through the MEK-ERK signaling pathway in Hazaks' esophageal squamous cell carcinoma

    摘要
  • 摘要:
      目的  探讨Lrig1、NF-κB及MMP-2在哈萨克族食管癌患者中的表达与PI3K-AKT、MEK-ERK信号通路及与临床病理指标之间的关系。
      方法  采用RT-PCR方法检测48例哈萨克族食管癌患者组织中Lrig1、NF-κB、MMP-2表达, 探讨NF-κB、MMP-2的表达与Lrig1的关系。纳入PI3K及MEK信号通路的关键基因PI3K、AKT1、MEK1、MEK2、MEK5、ERK1、ERK2, 研究Lrig1调控NF-κB、MMP-2基因表达的通路。
      结果  转录水平mRNA检测显示在肿瘤组织及远端正常组织中NF-κB（P < 0.001, P= 0.014）、MMP-2（P=0.003, P=0.045）的表达与Lrig1 mRNA表达相关; 蛋白水平Western blot检测显示Lrig1与NF-κB、MMP-2可能呈负相关。在肿瘤组织中MEK5（P < 0.001）及ERK2（P=0.009）的表达与Lrig1相关; PI3K在正常组织中（P < 0.001）的表达与Lrig1相关。Lrig1、MMP-2及NF-κB协同表达率为52.1%（25/48）, 其协同表达与淋巴结转移相关（P=0.020）。
      结论  NF-κB、MMP-2与Lrig1协同表达促进食管癌患者的淋巴结转移, Lrig1可能是通过MEK-ERK信号通路调控相关基因表达。

     

    Abstract:
      Objective  This work aimed to investigate the expression levels of Lrig1, NF-κB, and MMP-2 in Hazak's esophageal squamous cell carcinoma (ESCC), as well as to explore the relationship of Lrig1 expression through the PI3K-AKT and MEK-ERK signaling pathways with clinicopathological indices.
      Methods  First, RT-PCR analysis was performed to study the expression levels of Lrig1, NF-κB, and MMP-2 in 48 ESCC and noncancerous specimens. Second, some key genes of the PI3K and MEK signaling pathways such as PI3K, AKT1, MEK1, MEK2, MEK5, ERK1, and ERK2 were detected in Lrig1-positive and -negative tissues to identify the possible signaling pathway of Lrig1-regulated NF-κB and MMP-2 expression.
      Results  The expression of NF-κB (P < 0.001, P=0.014) and MMP-2 (P=0.003, P=0.045) was significantly correlated with Lrig1 in cancer and distal normal tissues. Lrig1 may be negatively correlated with NF-κB and MMP-2 at the protein level. MEK5 (P < 0.001) and ERK2 (P=0.009) expression was associated with Lrig1 in cancer tissues. PI3K expression was correlated with Lrig1 in the distal normal tissues (P < 0.001). The coordinate expression ratio of Lrig1 to NF-κB and MMP2 reached 52.1% (25/48). This co-expression may be related to lymph node metastasis (P=0.020) but not to other clinicopathological features.
      Conclusions  These findings suggest that MMP-2 and NF-κB expression is related to Lrig1 in Hazak's ESCC, and that MEK and ERK2 mRNAexpression are related to Lrig1. The co-expression of NF-κB, MMP-2, and Lrig1 may promote lymph node metastasis in ESCC among Hazak people. Lrig1 may regulate the expression of target genes not through the PI3K-AKT pathway but through the MEK-ERK signaling pathway. Further related studies are needed in the future.

     

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