Abstract:
Objective This work aimed to investigate the expression level of lysophosphatidic acid(LPA) and its receptors.The paper also discussed the interrelationship among the LPA, the receptors, and IL-6 and IL-8 in breast cancer tissues.
Methods The expressions of the 3 hypo-types of LPA receptor in the breast cancer and paraneoplastic tissues were detected using semi-quantitative reverse transcription polymerase chain reaction.The plasma levels of LPA, IL-6 and IL-8 were respectively detected in healthy subjects and in patients with benign breast tumor using the LPA biochemistry and enzyme linked immunosorbent assay kits.
Results The plasma LPA level was significantly higher in patients with breast cancer metastasis than in those with local breast cancer(P < 0.01), benign breast tumor(P < 0.01), and healthy volunteers(P < 0.01).In addition, the IL-6 and IL-8 plasma levels were higher in the group with metastasis compared with the other three groups, too(P < 0.01).LPA1 expression level was higher in breast cancer tissue than in benign breast tumor(P < 0.05) and in normal breast tissue(P < 0.05).There was a significantly positive correlation between the plasma LPA and the plasma IL-6 in patients with breast cancer(P < 0.01), and between the plasma LPA and IL-8(P < 0.01).
Conclusion LPA expressions on the endogenous IL-6 and IL-8 in patients with breast cancer may have an up-regulation.Moreover, the detection of the LPA, IL-6, and IL-8 expression levels may have some predictable effects on metastatic breast cancer, especially bone metastases.
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