食管癌组织VEGF-C SDF-1/CXCR4的表达及其与淋巴结转移关系的研究

李幼梅; 祝淑钗; 沈文斌; 李娟; 李曙光; 苏景伟; 刘志坤

doi:10.3969/j.issn.1000-8179.2013.11.007

食管癌组织VEGF-C SDF-1/CXCR4的表达及其与淋巴结转移关系的研究

Expression of VEGF-C, SDF-1, and CXCR4 in esophageal carcinoma and its relationship with lymph node metastasis

摘要

摘要:
目的研究VEGF-C、SDF-1及CXCR4在食管鳞癌中的表达, 并分析其与食管癌相关临床病理因素的关系和对预后的影响。
方法通过免疫组织化学SP法检测VEGF-C、SDF-1及CXCR4在95例食管癌切除组织中的表达, 并探讨其临床意义。
结果 VEGF-C及SDF-1、CXCR4三种因子蛋白表达均主要位于肿瘤细胞的胞质, 阳性率分别为89.5%、84.2%、69.5%, 其中CXCR4在肿瘤细胞中未见到强阳性表达。VEGF-C和SDF-1在肿瘤细胞的表达与肿瘤细胞分化程度、浸润深度、淋巴结转移和转移的个数以及病理TNM分期均有相关性, 其中有淋巴结转移组的VEGF-C和SDF-1表达率均高于无淋巴结转移组, 且差异均有统计学意义(χ²=6.319, P=0.012;χ²=5.821, P=0.016)。而CXCR4在肿瘤细胞的表达仅与肿瘤细胞分化程度相关, 且分化越高表达越强。多因素分析显示淋巴结转移、淋巴结转移数目、病理TNM分期及SDF-1蛋白表达为影响预后的独立性因素(P < 0.05)。
结论食管鳞癌组织中VEGF-C、SDF-1的表达与多项临床病理指标尤其是淋巴结转移程度有关, 其中SDF-1可作为淋巴结转移及预后的免疫病理学指标, 同时VEGF-C与SDF-1/CXCR4在淋巴结转移中可能有协同作用。

Abstract:
Objective This work aims to investigate the expressions of vascular endothelial growth factor-c(VEGF-C), stromal cell-derived factor-1(SDF-1), and human chemokine(C-X-C motif) receptor 4(CXCR4) in esophageal squamous cell carcinoma.In addition, their correlation with clinicopathological factors and their effect on the prognostic value are also investigated.
Methods The immunohistochemical expressions of VEGF-C, SDF-1, and CXCR4, the relationship among them, and the clinicopathological factors including nodal metastasis and prognostic value in the surgical specimens from 95 patients with primary esophageal squamous cell carcinoma were assayed.
Results VEGF-C, SDF-1, and CXCR4 proteins were mainly located in the cytoplasm of the tumor cells.The positive expression rates of VEGF-C, SDF-1, and CXCR4 were 89.5%, 84.2%, and 69.5%, respectively.The positive expressions of VEGF-C and SDF-1 were significantly correlated with the cellular differentiation degree, tumor invasion depth, nodal metastasis, number of nodal metastasis, and the tumor, node, and metastasis(TNM) stage as defined by the World Health Organization.The positive rates of VEGF-C and SDF-1 expressions were higher in patients with lymph node metastasis than in those without lymph node.Significant differences were observed between the two(χ²=6.319, P=0.012;χ²=5.821, P=0.016).However, the positive expression of the CXCR4 protein was only correlated with the cellular differentiation degree.The Kaplan-Meier survival analysis results show the tumor invasion depth, lymph node metastasis, number of the metastasized lymph nodes, pathological TNM(pTNM) stage, and the expressions of VEGF-C, SDF-1, and CXCR4.The multivariate analysis results show that the tumor invasion depth, nodal metastasis, number of metastatic lymph nodes, pTNM stage, and expression of SDF-1 were independent prognostic factors.
Conclusion The expressions of VEGF-C and SDF-1 proteins exhibit a correlation with the clinicopathological variables, especially the lymph node metastasis.Evaluating the SDF-1 expression is useful to determine tumor characteristics, such as nodal metastasis and prognosis, in patients with esophageal squamous cell carcinoma.Moreover, VEGF-C and SDF-1/CXCR4 proteins may have a synergistic effect on the lymph node metastasis of esophageal squamous cell carcinoma.

HTML全文

参考文献(19)

施引文献

资源附件(0)