Abstract:
Objective This work aimed to investigate the feasibility of apoptosis imaging of Annexin V labeled with the radionuclide 153Sm.
Methods 153Sm-Annexin V was prepared by using the cyclic diethylene triamine pentaacetic acid (DTPA) method, after which physical, chemical, and biological assays of the protein were conducted.Apoptosis imaging experiment both for in vitro cell culture and for tumor-bearing mice was performed using 153Sm-Annexin V.
Results The features of 153Sm-Annexin V prepared via the cyclic DTPA method are as follows: colorless and transparent, pH 7.4, radioactivity of 100 μg/10 mCi/2 mL, radiochemical purity of over 90%, and 88.6% radiolabeling rate.Annexin V is sterile, pyrogen-free, and presents an LD50 of 333 μg/kg body weight and plasma half-life of 13 min.Positive apoptosis images were obtained from both cultured cells and tumor-bearing mice.
Conclusion 153Sm-Annexin V, as a radionuclide-labeled apoptosis imaging agent, exhibits a high labeling rate and relatively long half-life.The agent can efficiently facilitate continuous tracking and capturing of apoptotic peak activity, thus establishing an optimal apoptosis imaging window period.These advantages indicate that the proposed imaging method has great potential in many clinical applications.
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