刘义冰, 胡大利, 周雨朦, 黄绮玲, 潘诗云. 铜绿假单胞菌注射液对多种人恶性肿瘤细胞株的增殖抑制作用[J]. 中国肿瘤临床, 2013, 40(12): 694-697. DOI: 10.3969/j.issn.1000-8179.2013.12.003
引用本文: 刘义冰, 胡大利, 周雨朦, 黄绮玲, 潘诗云. 铜绿假单胞菌注射液对多种人恶性肿瘤细胞株的增殖抑制作用[J]. 中国肿瘤临床, 2013, 40(12): 694-697. DOI: 10.3969/j.issn.1000-8179.2013.12.003
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Yi-bing LIU, Da-li HU, Yu-meng ZHOU, Qi-ling HUANG, Shi-yun PAN. Pseudomonas aeruginosa injection inhibits proliferation of human carcinoma cell lines[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(12): 694-697. DOI: 10.3969/j.issn.1000-8179.2013.12.003
Citation: Yi-bing LIU, Da-li HU, Yu-meng ZHOU, Qi-ling HUANG, Shi-yun PAN. Pseudomonas aeruginosa injection inhibits proliferation of human carcinoma cell lines[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(12): 694-697. DOI: 10.3969/j.issn.1000-8179.2013.12.003
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铜绿假单胞菌注射液对多种人恶性肿瘤细胞株的增殖抑制作用

Pseudomonas aeruginosa injection inhibits proliferation of human carcinoma cell lines

    摘要
  • 摘要:
      目的   研究铜绿假单胞菌注射液(PA-MSHA) 在体外对人恶性肿瘤细胞(肝癌BEL-7402、乳腺癌MDA-MB-231、胃癌MKN-45、肺癌A549和结肠癌SW620) 生长状态的影响, 并进行PA-MSHA药物敏感度的比较和分析。
      方法  将PA-MSHA (1.8×109/mL) 进行梯度稀释, 在体外分别与5种肿瘤细胞共同培养72 h, 并在显微镜下观察细胞生长和形态变化。用MTT比色法检测细胞的增殖水平, 并计算IC50值。
      结果  镜下观察发现, PA-MSHA对5种肿瘤细胞的生长均有显著抑制。MTT法检测显示, PA-MSHA能够有效抑制肿瘤细胞的体外增殖, 并呈现出明显的药物剂量相关性。PA-MSHA对BEL-7402、MDA-MB-231、MKN-45、A549和SW620细胞的IC50值, 分别为2.12×108/mL、1.95×108/mL、2.09×108/mL、1.59×108/mL和1.32×108/mL。
      结论  铜绿假单胞菌注射液能够直接抑制人恶性肿瘤细胞的体外增殖, 并且对不同来源的肿瘤细胞具有广谱抑制作用。5种恶性肿瘤细胞对PA-MSHA的药物敏感性依次为: 结肠癌SW620 > 肺癌A549 > 乳腺癌MDA-MB-231 > 胃癌MKN-45 > 肝癌BEL-7402。

     

    Abstract:
      Objective   This work aimed to investigate the effect of Pseudomonas Aeruginosa Injection (PA-MSHA) on the growth of human carcinoma cell lines (hepatocellular carcinoma BEL-7402 cell, breast carcinoma MDA-MB-231 cell, gastric carcinoma MKN-45 cell, lung carcinoma A549 cell and colorectal carcinoma SW620) in vitro.The comparison and analysis of the PA-MSHA sensitivity was performed.
      Methods  The five human carcinoma cell lines were treated with Pseudomonas Aeruginosa Injection (PA-MSHA 1.8×109/mL) of gradient dilution for 72 h in vitro respectively, and observed by optical microscope.Methyl thiazolyl tetrazolium (MTT) was used to analyze cell proliferation, and linear fitting method to calculate IC50.
      Results  We found that PA-MSHA inhibited the growth of all of the carcinoma cells.The result of MTT showed dose-dependent inhibition effect of PA-MSHA on proliferation of carcinoma cells in vitro.IC50 of PA-MSHA was 2.12×108/ml (BEL-7402), 1.95×108/ml (MDA-MB-231), 2.09×108/ml (MKN-45), 1.59×108/ml (A549) and 1.32×108/ml (SW620), respectively.
      Conclusion  Pseudomonas Aeruginosa Injection directly inhibited proliferation of human carcinoma cells in vitro, and had inhibitory effect of broad spectrum on different tumor cell sources.Drug sensitivity of PA-MSHA in five carcinoma cell lines was SW620 > A549 > MDA-MB-231 > MKN-45 > BEL-7402.

     

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