miR-21与肾癌转移的相关性及其对肾癌细胞侵袭能力的影响

张辉; 郭艳; 尚超; 宋永胜

doi:10.3969/j.issn.1000-8179.2013.12.005

miR-21与肾癌转移的相关性及其对肾癌细胞侵袭能力的影响

Relationship between miR-21 and renal cancer metastasis and influence of miR-21 on the invasion ability of renal cancer cell

摘要

摘要:
目的探讨miR-21与肾癌转移的相关性, 及miR-21对肾癌Caki-1细胞侵袭能力的影响。
方法实时PCR检测原发未转移肾癌和原发伴转移肾癌组织中miR-21的表达。将miR-21的前体pre-miR-21和抑制物anti-miR-21分别转染肾癌Caki-1细胞, 实时PCR验证转染效果, 然后检测转染后细胞的侵袭能力。
结果与原发未转移肾癌相比, 原发伴转移肾癌组织中miR-21的表达显著上调; pre-miR-21和anti-miR-21转染后能够显著升高和降低Caki-1细胞miR-21的表达量; pre-miR-21组穿透滤膜的细胞数明显增加, 而anti-miR-21组穿透滤膜的细胞数明显减少。
结论 miR-21与肾癌的侵袭转移相关, miR-21能够促进肾癌细胞侵袭, 在肾癌中具有促进转移的作用。

Abstract:
Objective The present study aims to investigate the relationship between miR-21 and renal cancer metastasis as well as the influence of miR-21 on the invasiveness ability of renal cancer Caki-1 cells.
Methods Real-time PCR was performed to detect miR-21 in primary renal cancer without and with metastasis.Pre-miR-21 and anti-miR-21 were transfected to Caki-1 cells, respectively, and real-time PCR was used to detect transfection effects.Finally, invasiveness changes in Caki-1 cells were detected after transfection.
Results miR-21 expression in primary renal cancer with metastasis was much higher than that in primary renal cancer without metastasis.After transfection, pre-miR-21 and anti-miR-21 significantly increased and decreased the miR-21 expression in Caki-1 cells, respectively.The transmembrane cells of the pre-miR-21 group increased significantly, whereas those of the anti-miR-21 group decreased significantly.
Conclusion miR-21 and renal cancer metastasis are related.miR-21 advances renal cancer cell invasion and metastasis.

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