陈涛, 严立. 错配修复基因hMSH2和hMLH1在结直肠癌组织中的表达及临床意义[J]. 中国肿瘤临床, 2013, 40(12): 710-713. DOI: 10.3969/j.issn.1000-8179.2013.12.007
引用本文: 陈涛, 严立. 错配修复基因hMSH2和hMLH1在结直肠癌组织中的表达及临床意义[J]. 中国肿瘤临床, 2013, 40(12): 710-713. DOI: 10.3969/j.issn.1000-8179.2013.12.007
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Tao CHEN, Li YAN. Expression of mismatch repair genes hMLH1 and hMSH2 and their significance in patients with colorectal carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(12): 710-713. DOI: 10.3969/j.issn.1000-8179.2013.12.007
Citation: Tao CHEN, Li YAN. Expression of mismatch repair genes hMLH1 and hMSH2 and their significance in patients with colorectal carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(12): 710-713. DOI: 10.3969/j.issn.1000-8179.2013.12.007
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错配修复基因hMSH2和hMLH1在结直肠癌组织中的表达及临床意义

Expression of mismatch repair genes hMLH1 and hMSH2 and their significance in patients with colorectal carcinoma

    摘要
  • 摘要:
      目的   评价hMLH1和hMSH2蛋白在结直肠癌中的表达及意义。
      方法   选取2009年1月至2011年12月经病理学确诊的结肠癌手术切除标本72例, 所有患者在术前均未接受过放疗或化疗, 内镜活检取正常肠黏膜上皮25例。免疫组织化学检测hMLH1和hMSH2蛋白表达情况。
      结果   结直肠癌组织中hMSH2缺失率为88.9% (64/72), 高于正常肠组织中hMSH2蛋白缺失率28.0% (7/25)。hMSH2蛋白缺失率随T分期增加而增加, 但差异无统计学意义(χ2=37.622, P < 0.001);hMSH2蛋白缺失率与N分期相关, 有淋巴结转移者的hMSH2蛋白缺失40例, 缺失率达97.6% (40/41), 无淋巴结转移患者的hMSH2蛋白缺失率为77.4% (24/31, χ2=7.251, P=0.007)。而hMLH1蛋白缺失率为90.3% (65/72), 高于正常组肠组织中hMLH1蛋白缺失率为32.0% (8/25, χ2=33.847, P < 0.001), 但与肿瘤部位、分期、分化程度均无关。
      结论   在结直肠癌组织中存在错配修复基因hMSH2和hMLH1蛋白缺失, 且表达缺失与肿瘤分期有关。通过免疫组织化学方法检测hMLH1和hMSH2蛋白表达可以简便、准确地发现错配修复基因的突变, 从而对其后期的治疗和预后判断有参考价值。

     

    Abstract:
      Objective   The present study aimed to examine the expression of mismatch repair genes, namely, human mutL homolog 1 (hMLH1) and human mutL homolog 2 (hMSH2), in colorectal carcinoma, and to investigate the relationship of hMLH1 and hMSH2 expression with clinicopathological features.
      Methods   Seventy-two patients with pathologically colorectal carcinoma and underwent surgical resection were included in the study.All patients did not undergo preoperative chemotherapy or radiotherapy.Immunohistochemical method was used to examine the hMLH1 and hMSH2 expressions in the colorectal carcinoma.The correlation of hMLH1 and hMSH2 expression with pathological features was investigated.
      Results   The loss rate of hMSH2 expression in colorectal cancer tissue was higher than that in normal tissue (88.9% vs.28.0%, χ2=37.622, P < 0.001).The loss rate of hMSH2 expression increased with T staging, but without significant difference.The loss rate of hMSH2 expression in patients with lymph node metastases was 97.6%, whereas the rate was 77.4% in patients with no lymph node metastases.Moreover, the results were significant (χ2=7.251, P= 0.007).The loss rate of hMLH1 expression in colorectal cancer tissue was 90.3% (65/72).The loss rate of hMLH1 expression was not correlated with tumor site, tumor stage, and tumor grade.
      Conclusion   The combined detection of hMLH-1 and hMSH-2 expressions may be used to evaluate the prognosis of colorectal cancer.

     

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