Abstract:
Objective This study aimed to investigate the antiangiogenesis of valproic acid (VPA) in leukemia cell line-transplanted tumor in nude mice and to explore the mechanisms of VPA.
Methods A nude mice model with xenograft tumor was established by the subcutaneous inoculation of Kasumi-1 cells. The mice were randomly assigned to control and VPA-treated groups. CD34 expression was assessed by immunohistochemistry. Basic FGF mRNA and protein levels were quantified by RT-PCR, Western blot analysis, and immunohistochemistry.
Results After 2 weeks of treatment with VPA, in the VPA-treated group, CD34 expression and the calculated MVD markedly decreased (P < 0.01). After treatment with VPA, the mRNA (0.321 ± 0.046) and protein (0.493 ± 0.026) levels of bFGF also significantly reduced (P < 0.01) compared with the mRNA (0.151 ± 0.136; P < 0.01) and protein (0.298 ± 0.011; P < 0.01) levels of the control group.
Conclusion VPA induced the antiangiogenesis of the leukemia cell line-transplanted tumor in nude mice. The mechanism of action may include the inhibition of bFGF expression.