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摘要:
目的 探讨Golph3在结直肠癌组织中的表达及其与细胞凋亡的关系。 方法 免疫组织化学方法检测62例结直肠癌组织中的Golph3、Caspase-3的表达，TUNEL法检测结直肠癌组织的凋亡指数，分析结直肠癌组织中的Golph3表达与临床病理因素、细胞凋亡的相关性。 结果 Golph3在结直肠癌组织中的表达率明显高于正常结直肠黏膜（P < 0.05）；低分化、浸润深度侵及浆膜外、淋巴结转移、Ⅲ期的结直肠癌组织中Golph3的表达率分别明显高于高中分化、浆膜内、无淋巴结转移者、Ⅰ~Ⅱ期结直肠癌组织的表达率（P均 < 0.05），而Golph3的表达与年龄、性别、肿瘤位置、大体类型、肿瘤长度无关（P>0.05）。Golph3高表达的结直肠癌组织中Cas. pase-3阳性表达率及凋亡指数明显低于Golph3低表达的结直肠癌组织（P < 0.05），相关分析显示结直肠癌组织中Golph3的表达与AI呈负相关（r=-0.320，P < 0.05）。 结论 结直肠癌组织中Golph3存在过表达，与细胞凋亡呈负相关。 -
关键词:
- 结直肠肿瘤 /
- Golph3 /
- 免疫组织化学TUNEL /
- TUNEL /
- 凋亡
Abstract:Objective To investigate the correlation between the expression of Golgi phosphoprotein 3 (GOLPH3) and the occurrence of apoptosis in colorectal cancer cells (CRC). Methods Immunohistochemical assays of GOLPH3 and caspase-3 were performed on the paraffin-embedded sections of 62 CRC samples using the standard streptavidin-peroxidase technique. The apoptotic index of the CRCs was examined using the in situ terminal deoxynucleotidyl transferase nick-end labeling technique. The relationship of the GOLPH3 expression, the cell apoptosis, and the clinicopathologic parameters was analyzed. Results The positive rates of GOLPH3 expression were significantly higher in the CRC tissues (53.2%) than in the normal colorectal mucosa (37.2%; P < 0.05). Likewise, GOLPH3 expression was significantly higher in poorly differentiated cancer tissue, tissue outside the serous membrane, metastatic lymph node tissue, and the stage Ⅲ CRCs, as compared with those of the moderately to well differentiated tissue, tissue inside the serous membrane, lymph node tissue without metastasis, and the stage Ⅰ to Ⅱ CRCs (P < 0.05). However, GOLPH3 expression was not significantly correlated with the other clinicopathologic parameters, namely, the age and sex of the patients as well as the site, depth, and length of the invasive tumor (P>0.05). The caspase-3 expression and apoptotic index were significantly lower in the GOLPH3-positive CRC tissue than in the GOLPH3-negative tissue (P < 0.05). GOLPH3 expression was negatively correlated with the apoptotic index of CRCs based on the Spearman correlation (r=-0.320, P < 0.05). Conclusion GOLPH3 overexpression in CRC tissue is negatively correlated with apoptotic index. -
Key words:
- colorectal neoplasms /
- Golph3 /
- immunohistochemistry /
- TUNEL /
- apoptosis
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图 1 Golph3在结直肠癌组织中的表达（S-P×400）
Figure 1. Expression of Golph3 in colorectal cancer tissue(S-P×400)
图 2 Golph3在正常结直肠黏膜中的表达（S-P×400）
Figure 2. Expression of Golph3 in the normal colorectal mucosa(S-P×400)
图 3 aspase-3在结直肠癌组织中的表达（S-P×400）
Figure 3. Expression of caspase-3 in colorectal cancer tissue(S-P×400)
表 1 结直肠癌组织中Golph3表达与临床病理因素、Caspase-3表达的关系 例
Table 1. Relationship of Golph3 and caspase-3 expression in colorectal cancer tissues with the clinicopathologic factors (n)
表 2 结直肠癌组织中Golph3表达与AI的关系 x±s，%
Table 2. Relationship between the Golph3 expression and apoptotic index in colorectal cancer tissue x±s, %
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