力超, 林贤东, 蔡晓雯, 林建银, 郑雄伟. 凝血酶敏感素-2在胃癌中的表达及其临床意义[J]. 中国肿瘤临床, 2013, 40(20): 1227-1231. DOI: 10.3969/j.issn.1000-8179.20130241
引用本文: 力超, 林贤东, 蔡晓雯, 林建银, 郑雄伟. 凝血酶敏感素-2在胃癌中的表达及其临床意义[J]. 中国肿瘤临床, 2013, 40(20): 1227-1231. DOI: 10.3969/j.issn.1000-8179.20130241
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Chao LI, Xiandong LIN, Xiaonwen CAI, Jianyin LIN, Xiongwei ZHENG. Clinical significance of thrombospondin-2 expression in gastric carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(20): 1227-1231. DOI: 10.3969/j.issn.1000-8179.20130241
Citation: Chao LI, Xiandong LIN, Xiaonwen CAI, Jianyin LIN, Xiongwei ZHENG. Clinical significance of thrombospondin-2 expression in gastric carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2013, 40(20): 1227-1231. DOI: 10.3969/j.issn.1000-8179.20130241
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凝血酶敏感素-2在胃癌中的表达及其临床意义

Clinical significance of thrombospondin-2 expression in gastric carcinoma

    摘要
  • 摘要:
      目的  探讨胃癌中凝血酶敏感素-2（thrombospondin-2，THBS2）基因表达的临床意义及其与患者临床病理特征的关系，分析THBS2基因表达与胃癌组织中微血管密度（MVD）和基质金属蛋白酶-2（MMP-2）表达水平的相关性。
      方法  采用实时荧光定量PCR技术检测82例配对的胃癌组织及癌旁组织THBS2 mRNA的表达，并分析其与临床病理特征的关系；应用免疫组织化学Elivision法检测胃癌组织中MMP-2表达情况，通过CD34免疫组织化学染色评价肿瘤组织MVD；分析THBS2 mRNA的表达与MVD、MMP-2表达的相关性。
      结果  THBS2 mRNA在胃癌中的表达高于癌旁组织（P=0.002）；胃癌组织中THBS2 mRNA的表达水平与肿瘤浸润程度、MVD及MMP-2表达密切相关（P=0.023，r=0.35，P < 0.01，P=0.004），但与性别、年龄、肿物大小、组织分型及淋巴结转移无关（P=0.53，P=0.53，P=0.21，P=0.84，P=0.96）。
      结论  THBS2可能在胃癌发生和发展中起重要的作用；THBS2可能通过调节MMP-2的表达，促进肿瘤生长与转移，但其确切的作用及机制有待进一步的研究。

     

    Abstract:
      Objective   To investigate the clinical significance of thrombospondin-2 (THBS2) mRNA expression in gastric carcinoma and its relationships with clinicopathologic features, microvessel density (MVD), and matrix metalloproteinase-2 (MMP-2).
      Methods   THBS2 mRNA expression was detected in 82 cases of gastric carcinomas and adjacent tissues using real-time quantitative fluorescence polymerase chain reaction. The correlation of this expression with clinicopathologic features was also analyzed. Cluster of differentiation 34 (CD34) and MMP-2 protein expression was examined using an immunohistochemical Elivision method. MVD was determined based on CD34-positive tubular structures.
      Results   The THBS2 mRNA expression level was significantly higher in the gastric carcinomas than in paraneoplastic tissues (P=0.002). The expression was associated with the depth of tumor invasion, MVD, and MMP-2 (P=0.02, r= 0.35, P < 0.01, and P=0.004, respectively) but not with patient gender, patient age, tumor size, histological type, and lymph node metastasis (P=0.53, P=0.53, P=0.21, P=0.84, and P=0.96, respectively).
      Conclusions   THBS2 may be significantly involved in the occurrence and progression of gastric carcinoma. The effects of THBS2 on gastric tumor growth and metastasis can be monitored by controlling the MMP-2 expression in the carcinoma. However, the specific functions and underlying mechanisms of THBS2 require further investigation.

     

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