EphrinA1-Fc对人肾透明细胞癌786-O细胞EphA2和ERK表达影响的研究

徐金升; 白亚玲; 张俊霞; 崔立文; 张慧然; 张胜雷

doi:10.3969/j.issn.1000-8179.20130415

EphrinA1-Fc对人肾透明细胞癌786-O细胞EphA2和ERK表达影响的研究

Effect of EphrinA1-Fc on phosphorylation of EphA2 and ERK in 786-O renal carcinoma cells

摘要

摘要:
目的探讨EphrinA1-Fc对人肾透明细胞癌786-O细胞系促红细胞生成素产生肝细胞受体A2（EphA2）和细胞外调节蛋白激酶1/2（ERK1/2）磷酸化程度的影响。
方法应用可溶性配体EphrinA1-Fc干预人肾透明细胞癌786-O细胞系，采用Wsternblot方法分析不同时间点细胞内EphA2和ERK1/2的磷酸化程度。
结果 EphrinA1-Fc干预5、10、30、60 min后，p-EphA2、p-ERK的相对表达量逐渐增加（F=9.392，P=0.025；F=4.428，P=0.041），p-EphA2、p- ERK在EphrinA1-Fc干预前均未见表达。
结论 EphrinA1-Fc抑制肿瘤转移复发的可能机制之一是其促使肾透明细胞癌786-O细胞EphA2磷酸化而导致其降解实现。

Abstract:
Objective To detect the effect of EphrinA1-Fc on the phosphorylation of EphA2 and extracellular signal-regulated kinase (ERK) in 786-O renal carcinoma cells (RCCs).
Methods The soluble ligand EphrinA1-Fc was used to inhibit the 786-O RCCs in vitro. Western blot analysis was used to examine the phosphorylation of EphA2 and ERK1/2 in the 786-O RCCs at different time points.
Results After the intervention with EphrinA1-Fc for 5, 10, 30, and 60 min, the expression of p-EphA2 increased (F=9.392, P= 0.025) as well as that of p-ERK (F=4.428, P=0.041). No p-EphA2 and p-ERK expression was observed in the pre-intervention group.
Conclusion One of the possible mechanisms of the inhibitory effect of EphrinA1-Fc on tumor metastasis and recurrence involves thephosphorylation of EphA2 by EphrinA1-Fc, leading to the degradation of EphA2.

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